高强度超声(HIUS)后腹膜内加压气溶胶化疗(PIPAC)中阿霉素的组织渗透性增加。

IF 1.6 Q4 ONCOLOGY International Journal of Surgical Oncology Pub Date : 2019-12-12 eCollection Date: 2019-01-01 DOI:10.1155/2019/6185313
Veria Khosrawipour, Sören Reinhard, Alice Martino, Tanja Khosrawipour, Mohamed Arafkas, Agata Mikolajczyk
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引用次数: 11

摘要

背景:在过去的二十年里,高强度超声(HIUS)作为一种新的直接治疗实体瘤的治疗选择,以及一种更好的化疗递送和灌注方法,已被研究。据我们所知,这种治疗方法尚未在腹膜转移治疗中进行测试,腹膜内化疗的组织渗透有限一直是腹膜转移治疗的主要问题。液体滴注和加压气溶胶都受到这种限制的影响。本研究旨在评估HIUS是否能提高化疗的渗透率。方法:对新鲜死猪腹膜组织标本进行0、5、30、60、120和300秒的高强度超声检查。然后在12毫米汞柱和37°C的温度下,通过加压腹膜内气溶胶化疗(PIPAC)用阿霉素处理样品。使用荧光显微镜在冷冻薄片上测量阿霉素的组织渗透性。结果:HIUS 120秒后,观察到腹膜表面浅层肿胀引起的宏观结构变化。经HIUS治疗300秒的腹膜中,阿霉素的最大穿透率显著较高,穿透深度为962.88 ± 161.4μm(p<0.05)。不含HIUS的样品穿透深度为252.25 ± 60.41.组织穿透力随着HIUS持续时间的延长而显著增加,在HIUS治疗300秒后,穿透力增加了3.8倍。结论:HIUS可作为腹膜内化疗的腹膜组织制备方法。在不增加化疗浓度和使用短时间间隔防止组织结构损伤的情况下,可以实现更高的组织穿透率。需要进行更多的研究来分析HIUS与腹膜内化疗联合的效果。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Increased Tissue Penetration of Doxorubicin in Pressurized Intraperitoneal Aerosol Chemotherapy (PIPAC) after High-Intensity Ultrasound (HIUS).

Background: High-intensity ultrasound (HIUS) has been studied for the past two decades as a new therapeutic option for solid tumor direct treatment and a method for better chemotherapy delivery and perfusion. This treatment approach has not been tested to our knowledge in peritoneal metastatic therapy, where limited tissue penetration of intraperitoneal chemotherapy has been a main problem. Both liquid instillations and pressurized aerosols are affected by this limitation. This study was performed to evaluate whether HIUS improves chemotherapy penetration rates.

Methods: High-intensity ultrasound (HIUS) was applied for 0, 5, 30, 60, 120, and 300 seconds on the peritoneal tissue samples from fresh postmortem swine. Samples were then treated with doxorubicin via pressurized intraperitoneal aerosol chemotherapy (PIPAC) under 12 mmHg and 37°C temperature. Tissue penetration of doxorubicin was measured using fluorescence microscopy on frozen thin sections.

Results: Macroscopic structural changes, identified by swelling of the superficial layer of the peritoneal surface, were observed after 120 seconds of HIUS. Maximum doxorubicin penetration was significantly higher in peritoneum treated with HIUS for 300 seconds, with a depth of 962.88 ± 161.4 μm (p < 0.05). Samples without HIUS had a penetration depth of 252.25 ± 60.41. Tissue penetration was significantly increased with longer HIUS duration, with up to 3.8-fold increased penetration after 300 sec of HIUS treatment.

Conclusion: Our data indicate that HIUS may be used as a method to prepare the peritoneal tissue for intraperitoneal chemotherapy. Higher tissue penetration rates can be achieved without increasing chemotherapy concentrations and preventing structural damage to tissue using short time intervals. More studies need to be performed to analyze the effect of HIUS in combination with intraperitoneal chemotherapy.

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来源期刊
CiteScore
3.70
自引率
0.00%
发文量
5
审稿时长
20 weeks
期刊介绍: International Journal of Surgical Oncology is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies in all areas of surgical oncology.
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