成年囊性纤维化患者血浆鞘脂和总胆固醇水平降低。

Dilara Bal Topcu , Berrin Er , Filiz Ozcan , Mutay Aslan , Lutfi Coplu , Incilay Lay , Yesim Oztas
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引用次数: 0

摘要

背景:肺上皮中的鞘氨醇脂质对膜结构的连续性、囊泡运输和细胞存活起着关键作用。先前的研究报道鞘氨醇脂质在囊性纤维化的炎症病因中起作用。本研究的目的是研究成年囊性纤维化(CF)患者血浆鞘磷脂和神经酰胺的水平,并与健康对照组进行比较。材料和方法:从CF患者急性发作期(n=15)、出院期(n=13)和稳定期(n=11)采集血样。年龄相近的健康个体(n=15)作为对照。通过LC-MS/MS测量血浆中C16-C24鞘磷脂和C16-C24-神经酰胺的水平。此外,还测定了稳定期患者血浆样本中的胆固醇和甘油三酯水平。结果:除稳定期C16鞘磷脂水平外,CF患者所有时期的鞘磷脂和神经酰胺水平均显著低于健康对照组。然而,CF发作期、出院期和稳定期的血浆Cer和SM水平没有差异。CF患者的胆固醇水平明显低于健康人。我们发现胆固醇与C16鞘磷脂显著相关。结论:与健康对照组相比,我们观察到成年CF患者在病情恶化、出院和稳定期的血浆Cer和SM水平较低。在这三个时期,我们没有发现患者的Cer和SM水平之间有任何显著差异。我们有限的患者数量可能导致了这种统计上的微不足道。然而,与病情恶化水平相比,出院时SM16水平的百分比增加,而与病情恶化相比,稳定时Cer16和Cer20的百分比下降。在这样的随访研究中纳入更多的CF患者可以更好地证明恶化期、出院期和稳定期之间的任何可能差异。
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Decreased plasma levels of sphingolipids and total cholesterol in adult cystic fibrosis patients

Background

Sphingolipid species in the lung epithelium have a critical role for continuity of membrane structure, vesicular transport, and cell survival. Sphingolipid species were reported to have a role in the inflammatory etiology of cystic fibrosis by previous work. The aim of the study was to investigate the levels of plasma sphingomyelin and ceramide in adult cystic fibrosis (CF) patients and compared with healthy controls.

Materials and methods

Blood samples were obtained from CF patients at exacerbation (n = 15), discharge (n = 13) and stable periods (n = 11). Healthy individuals (n = 15) of similar age served as control. Levels of C16–C24 sphingomyelin and C16–C24 ceramide were measured in the plasma by LC-MS/MS. Also, cholesterol and triglyceride levels were determined in plasma samples of the patients at stable period.

Results

All measured sphingomyelin and ceramide levels in all periods of CF patients were significantly lower than healthy controls except C16 sphingomyelin level in the stable period. However, plasma Cer and SM levels among exacerbation, discharge, and stable periods of CF were not different. CF patients had significantly lower cholesterol levels compared to healthy individuals. We found significant correlation of cholesterol with C16 sphingomyelin.

Conclusion

We observed lower plasma Cer and SM levels in adult CF patients at exacerbation, discharge, and stable periods compared to healthy controls. We didn't find any significant difference between patient Cer and SM levels among these three periods. Our limited number of patients might have resulted with this statistical insignificance. However, percentage of SM16 levels were increased at discharge compared to exacerbation levels, while percentage of Cer16 and Cer 20 decreased at stable compared to exacerbation. Inclusion of a larger number of CF patients in such a follow up study may better demonstrate any possible difference between exacerbation, discharge, and stable periods.

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来源期刊
Prostaglandins, leukotrienes, and essential fatty acids
Prostaglandins, leukotrienes, and essential fatty acids Clinical Biochemistry, Endocrinology, Diabetes and Metabolism
CiteScore
5.30
自引率
0.00%
发文量
0
审稿时长
64 days
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