来自粪便样本的日本睡鼠物种鉴定和饮食分析的非侵入性遗传方法

IF 0.8 4区 生物学 Q3 ZOOLOGY Mammal Study Pub Date : 2023-08-11 DOI:10.3106/ms2023-0003
J. Sato, Haruna Matsuda, Honoka Fujita, Kouki Yasuda, Haruka Aiba, S. Minato
{"title":"来自粪便样本的日本睡鼠物种鉴定和饮食分析的非侵入性遗传方法","authors":"J. Sato, Haruna Matsuda, Honoka Fujita, Kouki Yasuda, Haruka Aiba, S. Minato","doi":"10.3106/ms2023-0003","DOIUrl":null,"url":null,"abstract":"Abstract. Noninvasive methods for species identification and dietary profiling from fecal samples in an artificial nest box were developed for the Japanese dormouse Glirulus japonicus. The species is a natural monument in Japan protected by domestic regulations since 1975. We assessed the mitochondrial cytochrome b gene for species identification and obtained location-specific sequences for Oki and Yamanashi, Japan. This marker was able to identify the dormouse species from feces. We also performed DNA metabarcoding analyses to clarify the invertebrate and plant diets of the Japanese dormouse. Various invertebrates and plants were detected, supporting the omnivorous nature of this species. Furthermore, almost all dietary items were identified to the genus level (82.1% and 99.5% for invertebrates and plants, respectively). The dietary components in Yamanashi Prefecture suggested that the Japanese dormouse mainly consumed Lepidoptera and various plants in summer, and Diptera and Hemiptera (aphids) invertebrates and Actinidia sp. plants in autumn. The latter plants produce fruit in autumn, enabling the dormouse to accumulate fat before hibernation. We discuss the potential and pitfalls of the noninvasive method, including the necessity of local DNA databases, reliability of the global DNA database, sampling procedure to avoid contamination, and individual identification.","PeriodicalId":49891,"journal":{"name":"Mammal Study","volume":" ","pages":""},"PeriodicalIF":0.8000,"publicationDate":"2023-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Noninvasive Genetic Methods for Species Identification and Dietary Profiling of the Japanese Dormouse Glirulus japonicus from Fecal Samples\",\"authors\":\"J. Sato, Haruna Matsuda, Honoka Fujita, Kouki Yasuda, Haruka Aiba, S. Minato\",\"doi\":\"10.3106/ms2023-0003\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Abstract. Noninvasive methods for species identification and dietary profiling from fecal samples in an artificial nest box were developed for the Japanese dormouse Glirulus japonicus. The species is a natural monument in Japan protected by domestic regulations since 1975. We assessed the mitochondrial cytochrome b gene for species identification and obtained location-specific sequences for Oki and Yamanashi, Japan. This marker was able to identify the dormouse species from feces. We also performed DNA metabarcoding analyses to clarify the invertebrate and plant diets of the Japanese dormouse. Various invertebrates and plants were detected, supporting the omnivorous nature of this species. Furthermore, almost all dietary items were identified to the genus level (82.1% and 99.5% for invertebrates and plants, respectively). The dietary components in Yamanashi Prefecture suggested that the Japanese dormouse mainly consumed Lepidoptera and various plants in summer, and Diptera and Hemiptera (aphids) invertebrates and Actinidia sp. plants in autumn. The latter plants produce fruit in autumn, enabling the dormouse to accumulate fat before hibernation. We discuss the potential and pitfalls of the noninvasive method, including the necessity of local DNA databases, reliability of the global DNA database, sampling procedure to avoid contamination, and individual identification.\",\"PeriodicalId\":49891,\"journal\":{\"name\":\"Mammal Study\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":0.8000,\"publicationDate\":\"2023-08-11\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Mammal Study\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.3106/ms2023-0003\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"ZOOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Mammal Study","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.3106/ms2023-0003","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"ZOOLOGY","Score":null,"Total":0}
引用次数: 0
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Noninvasive Genetic Methods for Species Identification and Dietary Profiling of the Japanese Dormouse Glirulus japonicus from Fecal Samples
Abstract. Noninvasive methods for species identification and dietary profiling from fecal samples in an artificial nest box were developed for the Japanese dormouse Glirulus japonicus. The species is a natural monument in Japan protected by domestic regulations since 1975. We assessed the mitochondrial cytochrome b gene for species identification and obtained location-specific sequences for Oki and Yamanashi, Japan. This marker was able to identify the dormouse species from feces. We also performed DNA metabarcoding analyses to clarify the invertebrate and plant diets of the Japanese dormouse. Various invertebrates and plants were detected, supporting the omnivorous nature of this species. Furthermore, almost all dietary items were identified to the genus level (82.1% and 99.5% for invertebrates and plants, respectively). The dietary components in Yamanashi Prefecture suggested that the Japanese dormouse mainly consumed Lepidoptera and various plants in summer, and Diptera and Hemiptera (aphids) invertebrates and Actinidia sp. plants in autumn. The latter plants produce fruit in autumn, enabling the dormouse to accumulate fat before hibernation. We discuss the potential and pitfalls of the noninvasive method, including the necessity of local DNA databases, reliability of the global DNA database, sampling procedure to avoid contamination, and individual identification.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Mammal Study
Mammal Study ZOOLOGY-
CiteScore
1.70
自引率
20.00%
发文量
23
审稿时长
>12 weeks
期刊介绍: Mammal Study is the official journal of the Mammal Society of Japan. It publishes original articles, short communications, and reviews on all aspects of mammalogy quarterly, written in English.
期刊最新文献
Rediscovery of type specimens of Nesorhinus hayasakai (Mammalia, Rhinocerotidae) from the Pleistocene of Taiwan Invasive Raccoons (Procyon lotor) have Little Effect on the Food Habits of Native Raccoon Dogs (Nyctereutes procyonoides) in a Satoyama Area of Tokyo Tandem Sleep: A Novel Behavior in Melon-Headed Whales The Food Habits of the Eurasian Otter Lutra lutra in South Korea Pre- and Postpartum Acoustic Activity in Captive Pacific White-Sided Dolphin (Lagenorhynchus obliquidens) Mothers
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1