Paraburkholderia sp. pfn29深层发酵生产聚羟基烷烃酸酯(PHAs)及优化

IF 2.3 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Electronic Journal of Biotechnology Pub Date : 2022-03-01 DOI:10.1016/j.ejbt.2021.12.003

Thayat Sriyapai , Thitima Chuarung , Kazuhide Kimbara , Siritron Samosorn , Pichapak Sriyapai

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引用次数: 12

摘要

聚羟基丁酸酯(PHB)和聚羟基丁酸酯-羟基戊酸酯(PHBV)是最常见的聚羟基烷酸酯(PHAs)。它们是在不平衡生长条件下以细胞内颗粒形式积聚在细胞质中的可生物降解塑料的重要候选者。本研究首次对Paraburkholderia sp. PFN29进行了深层发酵生产pha的研究。结果从泰国土壤样品中分离到200株细菌，其中6株细菌采用尼罗红染色法富集PHB。经16S rDNA基因测序鉴定，6株分离菌株与固氮菌属、Sphingobium属和Paraburkholderia属亲缘关系较近。PHB浓度最高的菌株为Paraburkholderia sp. PFN29(5±0.17 g/L)。在含有3% (w/v)葡萄糖和0.1% (w/v) (NH4)2Fe(SO4)的改性培养基中，在pH 7.0、35℃、200 rpm下振荡96 h，获得了PHB积累的最佳条件。在PHBV的初步合成中，利用PFN29从葡萄糖和有机酸(乙酰丙酸和丙酸)合成PHBV。1H核磁共振(1H NMR)光谱和气相色谱-质谱(GC/MS)分析证实了提取的相has与标准PHB和PHBV的比较。结论在5.14±0.17 g/L CDW条件下，spfn29可制备PHB, PHB含量可达97.3%。通过优化生产工艺，可以进一步提高PHBV的浓度。本研究为拟aburkholderia生产PHA提供了有用的数据，它可能被用作商业生产PHA的候选物种。如何引用esriyapai T, Chuarung T, Kimbara K等。Paraburkholderia sp. pfn29深层发酵生产聚羟基烷烃酸酯(PHAs)及优化中国生物医学工程学报(英文版);2012;https://doi.org/10.1016/j.ejbt.2021.12.003

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Production and optimization of polyhydroxyalkanoates (PHAs) from Paraburkholderia sp. PFN 29 under submerged fermentation

Background

Polyhydroxybutyrate (PHB) and polyhydroxybutyrate-co-hydroxyvalerate (PHBV) are the most common polyhydroxyalkanoates (PHAs). They are important candidates for biodegradable plastics that accumulate in the cytoplasm in the form of intracellular granules under unbalanced growth conditions. The present study focused on the production and optimization of PHAs from Paraburkholderia sp. PFN29 for the first time under submerged fermentation.

Results

Two hundred bacterial isolates were collected from soil samples in Thailand, six of which were found to accumulate PHB using the Nile red staining method. Six isolates were identified by 16S rDNA gene sequencing and were shown to be closely related to the genera Azotobacter, Sphingobium and Paraburkholderia. The best strain, Paraburkholderia sp. PFN29, showed the highest PHB concentration (5 ± 0.17 g/L). Optimization of PHB accumulation was achieved in modified medium containing 3% (w/v) glucose, and 0.1% (w/v) (NH₄)₂Fe(SO₄) with incubation at pH 7.0 and 35°C for 96 h with shaking at 200 rpm. In preliminary PHBV synthesis, PFN29 was utilized to synthesize PHBV from glucose and organic acids (levulinic acid and propionic acid). ¹H nuclear magnetic resonance (¹H NMR) spectroscopy and gas chromatography-mass spectrometry (GC/MS) analyses confirmed the extracted PHAs compared to standard PHB and PHBV.

Conclusions

PFN29 can be used for feasible PHB production with a PHB content of up to 97.3% of 5.14 ± 0.17 g/L CDW. The PHBV concentration can be further improved by optimizing the production parameters as cosubstrates. The present study provides useful data on PHA production by Paraburkholderia, which may be used as a candidate species for commercial PHA production.

How to cite

Sriyapai T, Chuarung T, Kimbara K, et al. Production and optimization of polyhydroxyalkanoates (PHAs) from Paraburkholderia sp. PFN 29 under submerged fermentation. Electron J Biotechnol 2022;56. https://doi.org/10.1016/j.ejbt.2021.12.003

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来源期刊

Electronic Journal of Biotechnology 工程技术-生物工程与应用微生物

CiteScore

5.60

自引率

0.00%

发文量

审稿时长

2 months

期刊介绍： Electronic Journal of Biotechnology is an international scientific electronic journal, which publishes papers from all areas related to Biotechnology. It covers from molecular biology and the chemistry of biological processes to aquatic and earth environmental aspects, computational applications, policy and ethical issues directly related to Biotechnology. The journal provides an effective way to publish research and review articles and short communications, video material, animation sequences and 3D are also accepted to support and enhance articles. The articles will be examined by a scientific committee and anonymous evaluators and published every two months in HTML and PDF formats (January 15th , March 15th, May 15th, July 15th, September 15th, November 15th). The following areas are covered in the Journal: • Animal Biotechnology • Biofilms • Bioinformatics • Biomedicine • Biopolicies of International Cooperation • Biosafety • Biotechnology Industry • Biotechnology of Human Disorders • Chemical Engineering • Environmental Biotechnology • Food Biotechnology • Marine Biotechnology • Microbial Biotechnology • Molecular Biology and Genetics •Nanobiotechnology • Omics • Plant Biotechnology • Process Biotechnology • Process Chemistry and Technology • Tissue Engineering