Bhavya Khilnani, Kimberly Leon, Christopher Pino, Seth Drake, Robin Shandas, Steven R. Lammers
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引用次数: 0
摘要
基于挤出的生物打印(EBB)为水凝胶的3D打印提供了独特的优势,包括优越的结构完整性,高细胞密度和连续沉积。尽管有这些优点,印刷结构体缺乏机械稳定性限制了使用EBB制造高纵横比结构体。由于这一限制,模式化宏观支架以模仿高纵横比的解剖结构仍然是一个挑战。例如,在多次打印过程中,如果不使用复杂的牺牲材料,EBB很难打印具有可观长宽比的中空管。通过使用重力作为第5维输入(X, Y, Z,时间,重力),我们能够生成高纵横比结构,而无需3D打印机机制的进一步复杂化。使用重力辅助的生物打印方法,我们通常可以获得比没有重力辅助的类似结构打印的两倍以上的纵横比。
Gravity-assisted 3D bioprinting: Using gravity as a design input to build high aspect-ratio structures
Extrusion-based bioprinting (EBB) offers unique advantages for 3D printing of hydrogels including superior structural integrity, high cell density, and continuous deposition. Despite these advantages, the lack of mechanical stability of printed constructs limits the fabrication of high aspect ratio constructs using EBB. Due to this limitation, patterning macroscopic scaffolds to mimic anatomical structures of a high aspect ratio remains a challenge. For example, it is difficult for EBB to print hollow tubes of an appreciable length-to-width ratio without using complicated sacrificial materials in a multi-print process. By using gravity as a 5th-dimensional input (X, Y, Z, time, gravity), we were able to generate high aspect ratio structures without further complication of 3D printer mechanics. Using gravity-assisted bioprinting methods we routinely achieved aspect ratios greater than two times those of similar constructs printed without gravity assistance.
期刊介绍:
Bioprinting is a broad-spectrum, multidisciplinary journal that covers all aspects of 3D fabrication technology involving biological tissues, organs and cells for medical and biotechnology applications. Topics covered include nanomaterials, biomaterials, scaffolds, 3D printing technology, imaging and CAD/CAM software and hardware, post-printing bioreactor maturation, cell and biological factor patterning, biofabrication, tissue engineering and other applications of 3D bioprinting technology. Bioprinting publishes research reports describing novel results with high clinical significance in all areas of 3D bioprinting research. Bioprinting issues contain a wide variety of review and analysis articles covering topics relevant to 3D bioprinting ranging from basic biological, material and technical advances to pre-clinical and clinical applications of 3D bioprinting.