食品中掺入转基因大豆、玉米、动物肉类和莱克多巴胺在不同食品中的残留

IF 2.3 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Electronic Journal of Biotechnology Pub Date : 2022-01-01 DOI:10.1016/j.ejbt.2021.11.005
Amr A. Mostafa , Abd El-Hay G. Abu-Hassiba , Mariam T. ElRouby , Fatma Abou-Hashim , Hanaa S. Omar
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引用次数: 5

摘要

世界各国政府已经制定了各种战略来解决长期存在的粮食危机。受转基因生物污染的食品和经过激素处理的动物的肉类残留物最近受到越来越多的关注,对消费者构成严重的健康风险。本研究的目的是检测转基因玉米、大豆和水果中的重组DNA。此外,通过定性和定量方法检测了进口和本地食品中掺假的不同动物肉类和莱克多巴胺残留。结果从埃及不同超市、当地市场、街头小贩和贫民窟采集了60份本地和进口食品样本。结果表明,在25份样品中检测到重组DNA靶向序列,其中16份样品中存在共同调控基因(CaMV35s)。在玉米和大豆样品中均检测到Bt-11和RRS基因。然而,其中35份采用定性实时荧光定量PCR和酶联免疫吸附法(ELISA)进行RAC残留检测,对不同动物品种的肉类进行掺假筛选。结果显示,11份猪肉样品呈阳性掺假,6份肉类样品呈阳性掺假(狗、驴、猪肉、马、羊、鸡、大豆)。最后,在三种阳性掺假猪肉中检测出猪油。结论:根据国际法规,为了保护消费者免受食品掺假造成的危害,各国必须承认并实施高度限制的标签制度,以及在国际认可的实验室进行常规分析的定性和/或定量方法。引用方式:Mostafa AA, Abu-Hussein AE-HG, ElRouby MT等。食品中掺入转基因大豆、玉米、动物肉类和莱克多巴胺在不同食品中的残留。中国生物医学工程学报(英文版);2009;16。https://doi.org/10.1016/j.ejbt.2021.11.005
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Food adulteration with genetically modified soybeans and maize, meat of animal species and ractopamine residues in different food products

Background

Governments around the world have developed a variety of strategies to address the long-standing food crisis. Food contaminated by genetically modified organisms (GMOs) and meat residues from hormonally treated animals, has recently received increased attention, posing serious health risks to consumers. The aims of this study are to detect recombinant DNA in genetically modified maize, soybeans, and fruits. Furthermore, meat adulteration by mixing meat from different animal species and ractopamine residues (RAC) in imported and local food products were detected using qualitative and quantitative methods.

Results

Sixty local and imported food samples were collected from different supermarkets, local markets, street vendors, and slum areas in Egypt. The results revealed that the recombinant DNA targeted sequences were detected in 25 samples, with the common regulatory genes (CaMV35s) found in 16 of them. The Bt-11 and RRS genes were both detected in maize and soybean samples. However, 35 were used for a screening of meat adulteration with meat from different animal species using qualitative real-time PCR and RAC residue detection using ELISA. The results revealed that 11 samples of pork were positively adulterated, and six samples of meat were positively adulterated (dog, donkey, pork, horse, sheep, chicken, and soybean). Finally, lard was detected in three positively adulterated porcine meats.

Conclusions

It is concluded that, as per the international regulations, in order to protect consumers from the harm caused by food adulteration, countries must recognize and implement highly restricted labelling systems, as well as qualitative and/or quantitative methods in routine analyses in internationally accredited laboratories.

How to cite: Mostafa AA, Abu-Hussein AE-HG, ElRouby MT, et al. Food adulteration with genetically modified soybeans and maize, meat of animal species and ractopamine residues in different food products. Electron J Biotechnol 2022;55. https://doi.org/10.1016/j.ejbt.2021.11.005

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来源期刊
Electronic Journal of Biotechnology
Electronic Journal of Biotechnology 工程技术-生物工程与应用微生物
CiteScore
5.60
自引率
0.00%
发文量
50
审稿时长
2 months
期刊介绍: Electronic Journal of Biotechnology is an international scientific electronic journal, which publishes papers from all areas related to Biotechnology. It covers from molecular biology and the chemistry of biological processes to aquatic and earth environmental aspects, computational applications, policy and ethical issues directly related to Biotechnology. The journal provides an effective way to publish research and review articles and short communications, video material, animation sequences and 3D are also accepted to support and enhance articles. The articles will be examined by a scientific committee and anonymous evaluators and published every two months in HTML and PDF formats (January 15th , March 15th, May 15th, July 15th, September 15th, November 15th). The following areas are covered in the Journal: • Animal Biotechnology • Biofilms • Bioinformatics • Biomedicine • Biopolicies of International Cooperation • Biosafety • Biotechnology Industry • Biotechnology of Human Disorders • Chemical Engineering • Environmental Biotechnology • Food Biotechnology • Marine Biotechnology • Microbial Biotechnology • Molecular Biology and Genetics •Nanobiotechnology • Omics • Plant Biotechnology • Process Biotechnology • Process Chemistry and Technology • Tissue Engineering
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