Amr A. Mostafa , Abd El-Hay G. Abu-Hassiba , Mariam T. ElRouby , Fatma Abou-Hashim , Hanaa S. Omar
{"title":"食品中掺入转基因大豆、玉米、动物肉类和莱克多巴胺在不同食品中的残留","authors":"Amr A. Mostafa , Abd El-Hay G. Abu-Hassiba , Mariam T. ElRouby , Fatma Abou-Hashim , Hanaa S. Omar","doi":"10.1016/j.ejbt.2021.11.005","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><p>Governments around the world have developed a variety of strategies to address the long-standing food crisis. Food contaminated by genetically modified organisms (GMOs) and meat residues from hormonally treated animals, has recently received increased attention, posing serious health risks to consumers. The aims of this study are to detect recombinant DNA in genetically modified maize, soybeans, and fruits. Furthermore, meat adulteration by mixing meat from different animal species and ractopamine residues (RAC) in imported and local food products were detected using qualitative and quantitative methods.</p></div><div><h3>Results</h3><p>Sixty local and imported food samples were collected from different supermarkets, local markets, street vendors, and slum areas in Egypt. The results revealed that the recombinant DNA targeted sequences were detected in 25 samples, with the common regulatory genes (<em>CaMV35s</em>) found in 16 of them. The Bt-11 and RRS genes were both detected in maize and soybean samples. However, 35 were used for a screening of meat adulteration with meat from different animal species using qualitative real-time PCR and RAC residue detection using ELISA. The results revealed that 11 samples of pork were positively adulterated, and six samples of meat were positively adulterated (dog, donkey, pork, horse, sheep, chicken, and soybean). Finally, lard was detected in three positively adulterated porcine meats.</p></div><div><h3>Conclusions</h3><p>It is concluded that, as per the international regulations, in order to protect consumers from the harm caused by food adulteration, countries must recognize and implement highly restricted labelling systems, as well as qualitative and/or quantitative methods in routine analyses in internationally accredited laboratories.</p><p><strong>How to cite:</strong> Mostafa AA, Abu-Hussein AE-HG, ElRouby MT, et al. Food adulteration with genetically modified soybeans and maize, meat of animal species and ractopamine residues in different food products. Electron J Biotechnol 2022;55. https://doi.org/10.1016/j.ejbt.2021.11.005</p></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"55 ","pages":"Pages 65-77"},"PeriodicalIF":2.3000,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0717345821000543/pdfft?md5=26289087963a80a0961bb6f8adf6ef18&pid=1-s2.0-S0717345821000543-main.pdf","citationCount":"5","resultStr":"{\"title\":\"Food adulteration with genetically modified soybeans and maize, meat of animal species and ractopamine residues in different food products\",\"authors\":\"Amr A. Mostafa , Abd El-Hay G. Abu-Hassiba , Mariam T. ElRouby , Fatma Abou-Hashim , Hanaa S. Omar\",\"doi\":\"10.1016/j.ejbt.2021.11.005\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Background</h3><p>Governments around the world have developed a variety of strategies to address the long-standing food crisis. Food contaminated by genetically modified organisms (GMOs) and meat residues from hormonally treated animals, has recently received increased attention, posing serious health risks to consumers. The aims of this study are to detect recombinant DNA in genetically modified maize, soybeans, and fruits. Furthermore, meat adulteration by mixing meat from different animal species and ractopamine residues (RAC) in imported and local food products were detected using qualitative and quantitative methods.</p></div><div><h3>Results</h3><p>Sixty local and imported food samples were collected from different supermarkets, local markets, street vendors, and slum areas in Egypt. The results revealed that the recombinant DNA targeted sequences were detected in 25 samples, with the common regulatory genes (<em>CaMV35s</em>) found in 16 of them. The Bt-11 and RRS genes were both detected in maize and soybean samples. However, 35 were used for a screening of meat adulteration with meat from different animal species using qualitative real-time PCR and RAC residue detection using ELISA. The results revealed that 11 samples of pork were positively adulterated, and six samples of meat were positively adulterated (dog, donkey, pork, horse, sheep, chicken, and soybean). Finally, lard was detected in three positively adulterated porcine meats.</p></div><div><h3>Conclusions</h3><p>It is concluded that, as per the international regulations, in order to protect consumers from the harm caused by food adulteration, countries must recognize and implement highly restricted labelling systems, as well as qualitative and/or quantitative methods in routine analyses in internationally accredited laboratories.</p><p><strong>How to cite:</strong> Mostafa AA, Abu-Hussein AE-HG, ElRouby MT, et al. Food adulteration with genetically modified soybeans and maize, meat of animal species and ractopamine residues in different food products. 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Food adulteration with genetically modified soybeans and maize, meat of animal species and ractopamine residues in different food products
Background
Governments around the world have developed a variety of strategies to address the long-standing food crisis. Food contaminated by genetically modified organisms (GMOs) and meat residues from hormonally treated animals, has recently received increased attention, posing serious health risks to consumers. The aims of this study are to detect recombinant DNA in genetically modified maize, soybeans, and fruits. Furthermore, meat adulteration by mixing meat from different animal species and ractopamine residues (RAC) in imported and local food products were detected using qualitative and quantitative methods.
Results
Sixty local and imported food samples were collected from different supermarkets, local markets, street vendors, and slum areas in Egypt. The results revealed that the recombinant DNA targeted sequences were detected in 25 samples, with the common regulatory genes (CaMV35s) found in 16 of them. The Bt-11 and RRS genes were both detected in maize and soybean samples. However, 35 were used for a screening of meat adulteration with meat from different animal species using qualitative real-time PCR and RAC residue detection using ELISA. The results revealed that 11 samples of pork were positively adulterated, and six samples of meat were positively adulterated (dog, donkey, pork, horse, sheep, chicken, and soybean). Finally, lard was detected in three positively adulterated porcine meats.
Conclusions
It is concluded that, as per the international regulations, in order to protect consumers from the harm caused by food adulteration, countries must recognize and implement highly restricted labelling systems, as well as qualitative and/or quantitative methods in routine analyses in internationally accredited laboratories.
How to cite: Mostafa AA, Abu-Hussein AE-HG, ElRouby MT, et al. Food adulteration with genetically modified soybeans and maize, meat of animal species and ractopamine residues in different food products. Electron J Biotechnol 2022;55. https://doi.org/10.1016/j.ejbt.2021.11.005
期刊介绍:
Electronic Journal of Biotechnology is an international scientific electronic journal, which publishes papers from all areas related to Biotechnology. It covers from molecular biology and the chemistry of biological processes to aquatic and earth environmental aspects, computational applications, policy and ethical issues directly related to Biotechnology.
The journal provides an effective way to publish research and review articles and short communications, video material, animation sequences and 3D are also accepted to support and enhance articles. The articles will be examined by a scientific committee and anonymous evaluators and published every two months in HTML and PDF formats (January 15th , March 15th, May 15th, July 15th, September 15th, November 15th).
The following areas are covered in the Journal:
• Animal Biotechnology
• Biofilms
• Bioinformatics
• Biomedicine
• Biopolicies of International Cooperation
• Biosafety
• Biotechnology Industry
• Biotechnology of Human Disorders
• Chemical Engineering
• Environmental Biotechnology
• Food Biotechnology
• Marine Biotechnology
• Microbial Biotechnology
• Molecular Biology and Genetics
•Nanobiotechnology
• Omics
• Plant Biotechnology
• Process Biotechnology
• Process Chemistry and Technology
• Tissue Engineering