{"title":"三维生物打印GelMA平台用于生产肺肿瘤球体","authors":"Simona Villata , Marta Canta , Désirée Baruffaldi , Ignazio Roppolo , Candido Fabrizio Pirri , Francesca Frascella","doi":"10.1016/j.bprint.2023.e00310","DOIUrl":null,"url":null,"abstract":"<div><p>The study proposes a platform for the formation and culture of non-small cell lung cancer (NSCLC) spheroids, to obtain an <em>in vitro</em> model suitable for drug and therapy testing. To achieve that, traditional cell culture is compared to methacrylated gelatin (GelMA) 3D bioprinting, in order to explore not only the potential of the matrix itself, but also the impact of different architectures on spheroid formation. Starting from a systematic analysis, where GelMA concentration, methacrylation degree and cell seeding concentration is set; three different architectures (round, ring and grid) are analyzed in terms of spheroid formation and growth, using 3D bioprinting. The study reveals that Very High GelMA 7.5% w/v formulation, with single cells dispersed in, is the best bioink to obtain NSCLC spheroids. Moreover, grid architecture performs in the best way, because of the highest volume-surface area ratio. The designed GelMA platform can be used as a powerful <em>in vitro</em> tool for drug testing and therapy screening, that can be designed playing with four different parameters: cell concentration, GelMA methacrylation degree, GelMA concentration and geometry.</p></div>","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2023-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"3D bioprinted GelMA platform for the production of lung tumor spheroids\",\"authors\":\"Simona Villata , Marta Canta , Désirée Baruffaldi , Ignazio Roppolo , Candido Fabrizio Pirri , Francesca Frascella\",\"doi\":\"10.1016/j.bprint.2023.e00310\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>The study proposes a platform for the formation and culture of non-small cell lung cancer (NSCLC) spheroids, to obtain an <em>in vitro</em> model suitable for drug and therapy testing. To achieve that, traditional cell culture is compared to methacrylated gelatin (GelMA) 3D bioprinting, in order to explore not only the potential of the matrix itself, but also the impact of different architectures on spheroid formation. Starting from a systematic analysis, where GelMA concentration, methacrylation degree and cell seeding concentration is set; three different architectures (round, ring and grid) are analyzed in terms of spheroid formation and growth, using 3D bioprinting. The study reveals that Very High GelMA 7.5% w/v formulation, with single cells dispersed in, is the best bioink to obtain NSCLC spheroids. Moreover, grid architecture performs in the best way, because of the highest volume-surface area ratio. The designed GelMA platform can be used as a powerful <em>in vitro</em> tool for drug testing and therapy screening, that can be designed playing with four different parameters: cell concentration, GelMA methacrylation degree, GelMA concentration and geometry.</p></div>\",\"PeriodicalId\":37770,\"journal\":{\"name\":\"Bioprinting\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2023-09-25\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Bioprinting\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2405886623000532\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"Computer Science\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bioprinting","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2405886623000532","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"Computer Science","Score":null,"Total":0}
3D bioprinted GelMA platform for the production of lung tumor spheroids
The study proposes a platform for the formation and culture of non-small cell lung cancer (NSCLC) spheroids, to obtain an in vitro model suitable for drug and therapy testing. To achieve that, traditional cell culture is compared to methacrylated gelatin (GelMA) 3D bioprinting, in order to explore not only the potential of the matrix itself, but also the impact of different architectures on spheroid formation. Starting from a systematic analysis, where GelMA concentration, methacrylation degree and cell seeding concentration is set; three different architectures (round, ring and grid) are analyzed in terms of spheroid formation and growth, using 3D bioprinting. The study reveals that Very High GelMA 7.5% w/v formulation, with single cells dispersed in, is the best bioink to obtain NSCLC spheroids. Moreover, grid architecture performs in the best way, because of the highest volume-surface area ratio. The designed GelMA platform can be used as a powerful in vitro tool for drug testing and therapy screening, that can be designed playing with four different parameters: cell concentration, GelMA methacrylation degree, GelMA concentration and geometry.
期刊介绍:
Bioprinting is a broad-spectrum, multidisciplinary journal that covers all aspects of 3D fabrication technology involving biological tissues, organs and cells for medical and biotechnology applications. Topics covered include nanomaterials, biomaterials, scaffolds, 3D printing technology, imaging and CAD/CAM software and hardware, post-printing bioreactor maturation, cell and biological factor patterning, biofabrication, tissue engineering and other applications of 3D bioprinting technology. Bioprinting publishes research reports describing novel results with high clinical significance in all areas of 3D bioprinting research. Bioprinting issues contain a wide variety of review and analysis articles covering topics relevant to 3D bioprinting ranging from basic biological, material and technical advances to pre-clinical and clinical applications of 3D bioprinting.