Aqueous enzymatic extraction of Cornus wilsoniana fruit oil

The optimum enzyme of extracting oil from Cornus wilsoniana fruit was selected from xylanase,amylase,cellulase,neutral protease,composite plant hydrolytic enzymes and pectinase.The results showed that the oil extraction rate(65.29%) with cellulase was the highest among the six kinds of enzyme,and the oil exraction rate(64.05%) with composite plant hydrolytic enzymes was the second,the lowest oil extraction rate was 54.47% only by xylanase.By single factor test,the optimum conditions with cellulase was obtained as follows: pH 5.8,solid-liquid ratio 1∶3,enzyme dosage 2.5%,reaction temperature 40 ℃,reaction time 4 h.Under these conditions,the oil extraction rate reached 76.64%.