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Aqueous enzymatic extraction of Cornus wilsoniana fruit oil 水酶法提取山茱萸果油
Pub Date : 2012-10-01 DOI: 10.1109/BMSE.2012.6466186
J. Lijuan
The optimum enzyme of extracting oil from Cornus wilsoniana fruit was selected from xylanase,amylase,cellulase,neutral protease,composite plant hydrolytic enzymes and pectinase.The results showed that the oil extraction rate(65.29%) with cellulase was the highest among the six kinds of enzyme,and the oil exraction rate(64.05%) with composite plant hydrolytic enzymes was the second,the lowest oil extraction rate was 54.47% only by xylanase.By single factor test,the optimum conditions with cellulase was obtained as follows: pH 5.8,solid-liquid ratio 1∶3,enzyme dosage 2.5%,reaction temperature 40 ℃,reaction time 4 h.Under these conditions,the oil extraction rate reached 76.64%.
从木聚糖酶、淀粉酶、纤维素酶、中性蛋白酶、复合植物水解酶和果胶酶中筛选出了提取山茱萸果实油脂的最佳酶。结果表明:纤维素酶的提取率最高,为65.29%;复合植物水解酶的提取率次之,为64.05%;木聚糖酶的提取率最低,为54.47%;通过单因素试验,得到纤维素酶的最佳提取条件为:pH 5.8,料液比1∶3,酶用量2.5%,反应温度40℃,反应时间4 h,在此条件下,提取率可达76.64%。
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China Oils and Fats
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