龙猫睾丸、附睾和输精管的神经支配

W. Sienkiewicz, A. Szczurkowski, A. Dudek, J. Kaleczyc
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引用次数: 2

摘要

摘要以6只雄性龙猫为研究对象。用乙醚麻醉,腹腔注射尼布塔尔加深麻醉。以0.4 L 4%的多聚甲醛缓冲液经心灌注龙猫,收集其睾丸、附睾和输精管。取每只栗鼠一个睾丸的白膜染色为全载制备。将组织切成12 μm厚的低温切片,采用双免疫荧光法处理。在所有器官中,多巴胺β羟化酶阳性(DβH+)的神经纤维最为丰富。部分含有神经肽Y (NPY)。偶有npy阳性的神经纤维。单DβH+神经末梢也含有丙氨酸。少量供血器官的神经纤维进行P物质(SP)和降钙素基因相关肽(CGRP)染色。几乎所有的SP+纤维也是CGRP+,而单个CGRP+神经是sp免疫阴性的。部分神经末梢对囊泡型乙酰胆碱转运体和血管活性肠肽有免疫反应。所研究的器官神经支配不均匀。在靠近睾丸系膜边界的白膜区域神经密度最高,在性腺自由边界处神经密度逐渐减少。睾丸实质的维管组织除偶见提供精小管的DβH+神经外,均无神经纤维存在。附睾头部有中等数量的神经末梢,但在附睾体和附睾尾部神经数量逐渐增加。输精管有大量的神经纤维。输精管索段与腹段的神经支配密度无明显差异。
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Innervation of the chinchilla testis, epididymis, and vas deferens
Abstract The study was performed on six male chinchillas. The animals were anaesthetised with ether and the anaesthesia was deepened with nembuthal injected intraperitoneally. The chinchillas were then transcardially perfused with 0.4 L of 4% buffered paraformaldehyde, and testes, epididymides, and vasa deferentia were collected. The tunica albuginea from one testis from each chinchilla was stained as whole-mount preparation. The tissues were cut into 12 μm-thick cryostat sections, and processed for double-immunofluorescence method. In all organs studied, the most abundant nerve fibres were dopamine β hydroxylase positive (DβH+). Some of them contained neuropeptide Y (NPY). Sporadically NPY-positive-only nerve fibres were found. Single DβH+ nerve terminals contained also galanine. Small numbers of the nerve fibres supplying studied organs were stained for substance P (SP) and calitonin gene related peptide (CGRP). Almost all SP+ fibres were also CGRP+, whereas single CGRP+ nerves were SPimmunonegative. Some nerve terminals were immunoreactive to vesicular acetylcholine transporter and vasoactive intestinal peptide. The organs studied were innervated unevenly. The highest density of the nerves was found in the areas of the tunica albuginea adjacent to the mesorchial border of the testis and their number gradually decreased towards the free border of the gonad. None of the vascular tissue of the testicular parenchyma was free of the nerve fibres, except sporadically encountered DβH+ nerves which supply seminiferous tubules. Within the head of the epididymis a moderate number of nerve terminals were found, but in the body and tail of the organ the number of nerves gradually increased. The vas deferens was supplied with very numerous nerve fibres. There were no differences in the density of the innervation between the funicular and abdominal part of the vas deferens.
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