一种新型可降解PCL/PLLA带用于骨折内固定。

IF 4.2 3区 医学 Q2 ENGINEERING, BIOMEDICAL Journal of Materials Science: Materials in Medicine Pub Date : 2023-11-08 DOI:10.1007/s10856-023-06759-7
Baoyan Jin, Chongjing Zhang, Zeyuan Zhong, Zichen Liu, Zhenhua Zhang, Dejian Li, Min Zhu, Baoqing Yu
{"title":"一种新型可降解PCL/PLLA带用于骨折内固定。","authors":"Baoyan Jin,&nbsp;Chongjing Zhang,&nbsp;Zeyuan Zhong,&nbsp;Zichen Liu,&nbsp;Zhenhua Zhang,&nbsp;Dejian Li,&nbsp;Min Zhu,&nbsp;Baoqing Yu","doi":"10.1007/s10856-023-06759-7","DOIUrl":null,"url":null,"abstract":"<div><p>Early fracture fixation is the critical factor in fracture healing. Common internal fracture implants are made of metallic materials, which often affects the imaging quality of CT and MRI. Most patients will choose secondary surgery to remove the internal fixation implants, which causes secondary damage to them. The development of new degradable internal fracture implants has attracted more and more attention from orthopedic surgeons and researchers. Based on these problems, we improved the various properties of medical grade polycaprolactone (PCL) by adding poly(L-lactide) (PLLA). We produced PCL/PLLA strapping bands with different mass ratios by injection molding. We compared the mechanical properties, degradation properties, cell biocompatibility, bone marrow mesenchymal stem cells (BMSCs) adhesion, proliferation, osteogenic differentiation and fracture fixation effect of these strapping bands. The results showed that the tensile strength and yield force of the strapping bands increased with the increase of the content of PLLA. The addition of PLLA could significantly improve the mechanical strength in the early stage and accelerate the degradation rate of the strapping band. PCL/PLLA (80/20) strapping band had no significant cytotoxicity toward rBMSCs and could promote osteogenic differentiation of rBMSCs. The strapping band could ensure femoral fracture healing of beagles in 3 months and didn’t cause damage to the surrounding tissues and main organs. This study will provide some new insights into the biodegradable products of PCL/PLLA blends for internal fixation of fracture.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div><div><p>We produced novel degradable PCL/PLLA strapping bands with different mass ratios by injection molding. We tested the biological safety of the prepared internal fixation strapping bands for fracture, such as cell experiment in vitro and animal experiment, and studied the degradation behavior in vitro. The strapping bands could ensure femoral fracture healing of beagles. This study will provide some new insights into the biodegradable products of PCL/PLLA blends for internal fixation of fracture. <b>A</b> Immunofluorescence staining of rBMSCs (live cells: green; dead cells: red). <b>B</b> Young’s modulus change curve during strapping bands degradation. <b>C</b> The implantation process of strapping bands. <b>D</b> Micro-CT images of the beagle’s fracture recovery after the operation.</p></div></div></figure></div></div>","PeriodicalId":647,"journal":{"name":"Journal of Materials Science: Materials in Medicine","volume":null,"pages":null},"PeriodicalIF":4.2000,"publicationDate":"2023-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10632200/pdf/","citationCount":"0","resultStr":"{\"title\":\"A novel degradable PCL/PLLA strapping band for internal fixation of fracture\",\"authors\":\"Baoyan Jin,&nbsp;Chongjing Zhang,&nbsp;Zeyuan Zhong,&nbsp;Zichen Liu,&nbsp;Zhenhua Zhang,&nbsp;Dejian Li,&nbsp;Min Zhu,&nbsp;Baoqing Yu\",\"doi\":\"10.1007/s10856-023-06759-7\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Early fracture fixation is the critical factor in fracture healing. Common internal fracture implants are made of metallic materials, which often affects the imaging quality of CT and MRI. Most patients will choose secondary surgery to remove the internal fixation implants, which causes secondary damage to them. The development of new degradable internal fracture implants has attracted more and more attention from orthopedic surgeons and researchers. Based on these problems, we improved the various properties of medical grade polycaprolactone (PCL) by adding poly(L-lactide) (PLLA). We produced PCL/PLLA strapping bands with different mass ratios by injection molding. We compared the mechanical properties, degradation properties, cell biocompatibility, bone marrow mesenchymal stem cells (BMSCs) adhesion, proliferation, osteogenic differentiation and fracture fixation effect of these strapping bands. The results showed that the tensile strength and yield force of the strapping bands increased with the increase of the content of PLLA. The addition of PLLA could significantly improve the mechanical strength in the early stage and accelerate the degradation rate of the strapping band. PCL/PLLA (80/20) strapping band had no significant cytotoxicity toward rBMSCs and could promote osteogenic differentiation of rBMSCs. The strapping band could ensure femoral fracture healing of beagles in 3 months and didn’t cause damage to the surrounding tissues and main organs. This study will provide some new insights into the biodegradable products of PCL/PLLA blends for internal fixation of fracture.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div><div><p>We produced novel degradable PCL/PLLA strapping bands with different mass ratios by injection molding. We tested the biological safety of the prepared internal fixation strapping bands for fracture, such as cell experiment in vitro and animal experiment, and studied the degradation behavior in vitro. The strapping bands could ensure femoral fracture healing of beagles. This study will provide some new insights into the biodegradable products of PCL/PLLA blends for internal fixation of fracture. <b>A</b> Immunofluorescence staining of rBMSCs (live cells: green; dead cells: red). <b>B</b> Young’s modulus change curve during strapping bands degradation. <b>C</b> The implantation process of strapping bands. <b>D</b> Micro-CT images of the beagle’s fracture recovery after the operation.</p></div></div></figure></div></div>\",\"PeriodicalId\":647,\"journal\":{\"name\":\"Journal of Materials Science: Materials in Medicine\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":4.2000,\"publicationDate\":\"2023-11-08\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10632200/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Materials Science: Materials in Medicine\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s10856-023-06759-7\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"ENGINEERING, BIOMEDICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Materials Science: Materials in Medicine","FirstCategoryId":"5","ListUrlMain":"https://link.springer.com/article/10.1007/s10856-023-06759-7","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ENGINEERING, BIOMEDICAL","Score":null,"Total":0}
引用次数: 0

摘要

早期骨折固定是骨折愈合的关键因素。常见的内部骨折植入物由金属材料制成,这往往会影响CT和MRI的成像质量。大多数患者会选择二次手术来移除内固定植入物,这会对其造成二次损伤。新型可降解内骨折植入物的开发越来越受到骨科医生和研究人员的关注。基于这些问题,我们通过添加聚L-丙交酯(PLLA)来改善医用级聚己内酯(PCL)的各种性能。我们通过注射成型生产了具有不同质量比的PCL/PLLA捆扎带。我们比较了这些捆扎带的力学性能、降解性能、细胞生物相容性、骨髓间充质干细胞(BMSCs)粘附、增殖、成骨分化和骨折固定效果。结果表明,随着PLLA含量的增加,捆扎带的抗拉强度和屈服力都有所提高。PLLA的加入可以显著提高捆扎带早期的机械强度,加快捆扎带的降解速度。PCL/PLLA(80/20)条带对rBMSCs无明显的细胞毒性,可促进rBMSC的成骨分化。该绑带能保证比格犬股骨骨折在3个月内愈合,不会对周围组织和主要器官造成损伤。本研究将为PCL/PLLA共混物用于骨折内固定的生物可降解产品提供一些新的见解。我们通过注射成型生产了具有不同质量比的新型可降解PCL/PLLA捆扎带。我们对制备的骨折内固定带进行了体外细胞实验和动物实验等生物安全性测试,并研究了其体外降解行为。绑带可以保证比格犬股骨骨折的愈合。本研究将为PCL/PLLA共混物用于骨折内固定的生物可降解产品提供一些新的见解。rBMSC的免疫荧光染色(活细胞:绿色;死细胞:红色)。B捆扎带退化过程中的杨氏模量变化曲线。C捆扎带的植入过程。D比格犬手术后骨折恢复的显微CT图像。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

摘要图片

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
A novel degradable PCL/PLLA strapping band for internal fixation of fracture

Early fracture fixation is the critical factor in fracture healing. Common internal fracture implants are made of metallic materials, which often affects the imaging quality of CT and MRI. Most patients will choose secondary surgery to remove the internal fixation implants, which causes secondary damage to them. The development of new degradable internal fracture implants has attracted more and more attention from orthopedic surgeons and researchers. Based on these problems, we improved the various properties of medical grade polycaprolactone (PCL) by adding poly(L-lactide) (PLLA). We produced PCL/PLLA strapping bands with different mass ratios by injection molding. We compared the mechanical properties, degradation properties, cell biocompatibility, bone marrow mesenchymal stem cells (BMSCs) adhesion, proliferation, osteogenic differentiation and fracture fixation effect of these strapping bands. The results showed that the tensile strength and yield force of the strapping bands increased with the increase of the content of PLLA. The addition of PLLA could significantly improve the mechanical strength in the early stage and accelerate the degradation rate of the strapping band. PCL/PLLA (80/20) strapping band had no significant cytotoxicity toward rBMSCs and could promote osteogenic differentiation of rBMSCs. The strapping band could ensure femoral fracture healing of beagles in 3 months and didn’t cause damage to the surrounding tissues and main organs. This study will provide some new insights into the biodegradable products of PCL/PLLA blends for internal fixation of fracture.

Graphical Abstract

We produced novel degradable PCL/PLLA strapping bands with different mass ratios by injection molding. We tested the biological safety of the prepared internal fixation strapping bands for fracture, such as cell experiment in vitro and animal experiment, and studied the degradation behavior in vitro. The strapping bands could ensure femoral fracture healing of beagles. This study will provide some new insights into the biodegradable products of PCL/PLLA blends for internal fixation of fracture. A Immunofluorescence staining of rBMSCs (live cells: green; dead cells: red). B Young’s modulus change curve during strapping bands degradation. C The implantation process of strapping bands. D Micro-CT images of the beagle’s fracture recovery after the operation.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Journal of Materials Science: Materials in Medicine
Journal of Materials Science: Materials in Medicine 工程技术-材料科学:生物材料
CiteScore
8.00
自引率
0.00%
发文量
73
审稿时长
3.5 months
期刊介绍: The Journal of Materials Science: Materials in Medicine publishes refereed papers providing significant progress in the application of biomaterials and tissue engineering constructs as medical or dental implants, prostheses and devices. Coverage spans a wide range of topics from basic science to clinical applications, around the theme of materials in medicine and dentistry. The central element is the development of synthetic and natural materials used in orthopaedic, maxillofacial, cardiovascular, neurological, ophthalmic and dental applications. Special biomedical topics include biomaterial synthesis and characterisation, biocompatibility studies, nanomedicine, tissue engineering constructs and cell substrates, regenerative medicine, computer modelling and other advanced experimental methodologies.
期刊最新文献
Nano-titanium coating on glass surface to improve platelet-rich fibrin (PRF) quality Enhancing osteogenesis and mandibular defect repair with magnesium-modified acellular bovine bone matrix Shear bond strength between dental adhesive systems and an experimental niobium-based implant material Tauroursodeoxycholic acid combined with selenium accelerates bone regeneration in ovariectomized rats A particle-filled hydrogel based on alginate and calcium phosphate nanoparticles as bone adhesive
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1