金银花叶甾醇对某些医院感染性细菌的活性研究

D. Credo, F. Mabiki, F. Machumi, M. Chacha, C. Cornett
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摘要

在坦桑尼亚,Commiphora swynnertonii (Burtt)传统上被用于人类和动物的药用目的。此前,其叶提取物已被证明具有抗菌活性。然而,关于其抗菌活性的纯化合物的鉴定信息有限。因此,本研究从其叶提取物中分离纯化并评价其抗菌活性。对其二氯甲烷叶提取物进行柱层析,分离得到胆甾醇-7-en-3β-醇(1)和β-谷甾醇(2)两种甾醇。通过实验获得的核磁共振数据并与文献中获得的光谱数据进行比较,推断出它们的结构。测定甾醇对四种医院感染细菌的活性,即;采用肉汤微量稀释法测定金黄色葡萄球菌、粪肠球菌、大肠杆菌和铜绿假单胞菌的最低抑菌浓度(MIC)。胆甾醇-7-en-3β-醇(1)对金黄色葡萄球菌(MIC= 0.5 mg/ml)有较强的抑制活性,对其他细菌的抑制活性较弱(MIC >2mg/ml)。β-谷甾醇(2)对所有细菌均表现出弱活性(MIC >2mg/ml)。因此,在swynnertonii的叶子中存在抗菌活性化合物,如胆甾醇-7-en-3β-醇(1)等,可以证明其早期报道的抗菌活性,并进一步证实了该植物在治疗细菌感染方面的传统用途。
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Activity of Sterols Isolated from the Leaves of Commiphora swynnertonii (Burtt) Against Some Nosocomial Infectious Bacteria
Commiphora swynnertonii (Burtt) is traditionally used for medicinal purposes both in humans and animals in Tanzania. Previously, its leaves extract has been demonstrated antibacterial activity. However, there is limited information on identification of pure compounds responsible for its observed antibacterial activity. Therefore, this study was conducted to isolate and evaluate antibacterial activity of pure compounds isolated from its leaves extract. Colum chromatographic separation of its dichloromethane leaves extract afforded the isolation of two sterols namely, Cholest-7-en-3β-ol (1) and β-sitosterol (2). Their structures were deduced by using NMR data experimentally obtained and comparison with spectral data available in literatures. The sterols were evaluated for activity against four nosocomial infectious bacteria namely; Staphylococcus aureus, Enterococcus faecalis, Escherichia coli and Pseudomonas aeruginosa to determine Minimum Inhibitory Concentrations (MIC) by broth microdilution method. Cholest-7-en-3β-ol (1) demonstrated strong activity against S. aureus (MIC= 0.5 mg/ml) and weak activities (MIC >2mg/ml) against other bacteria. β-sitosterol (2) exhibited weak activities (MIC >2mg/ml) against all tested bacteria. Therefore, presence of antibacterial active compound(s) such as Cholest-7-en-3β-ol (1) among others in the leaves of C. swynnertonii can justify its earlier reported antibacterial activity and further confirms the claimed traditional uses of this plant in the treatment of bacterial infections.
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