利用羽状苔藓和长叶蓼提取物合成的绿色纳米铜抗真菌活性研究R

Hanisha R Hanisha R, Udayakumar R Udayakumar R, Selvayogesh S Selvayogesh S, Keerthivasan P Keerthivasan P, Gnanasekaran R Gnanasekaran R
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引用次数: 0

摘要

植物介导的纳米制造技术是纳米技术的一个新领域,由于其在安全性、成本效益、环境友好性和生物相容性方面的优势而受到传统方法的青睐。在目前的研究中,氯化铜和硫酸铜作为前体材料,研究了绿色合成的氧化铜纳米颗粒的有效性,这些纳米颗粒是用苔藓植物(brophyllum pinnatum)和长叶蓼(Polyalthia longifolia)合成的。对比研究了合成的氧化铜纳米颗粒对各种前驱体的效率。采用紫外可见分光光度计、X射线粉末衍射(XRD)、傅里叶变换红外光谱(FTIR)、扫描电镜(SEM)等不同的光谱和微观表征技术来证实氧化铜纳米颗粒的存在。以氯化铜和硫酸铜为前驱体的紫外可见分光光度计结果证实了氧化铜纳米粒子的存在,其吸收波长分别为235nm和575nm。X-射线衍射结果显示,颗粒的晶体结构在(111)、(200)和(220)处有三个峰,这证实了两种前驱体都存在氧化铜纳米颗粒。FTIR结果支持了几个官能团的存在,这些官能团参与了覆盖、还原和稳定氧化铜纳米粒子。SEM图像显示,所制备的氧化铜纳米颗粒呈球形,粒径在40 ~ 90 nm之间。进一步研究了合成的纳米颗粒对氯化铜和硫酸铜前驱体的抑菌活性。实验结果表明,100µg/ml对土曲半乳霉菌的最大抑制区分别为18mm和25mm。由于绿色合成的氧化铜纳米颗粒具有较高的生物活性和抗真菌活性,因此可以在植物病理学中用于对抗植物感染。
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Anti Fungal Activity of Green Synthesized Copper Nanoparticles Using Plant Extract of Bryophyllum Pinnatum (Lam.) and Polyalthia Longifolia (Sonn.) R
Plant-mediated nano-fabrication is a new area of nanotechnology that is preferred to traditional methods due to its advantages in terms of safety, cost efficiency, environmental friendliness, and biocompatibility. In the current study, copper chloride and copper sulphate are used as precursor materials to examine the effectiveness of green synthesized copper oxide nanoparticles using the plants Bryophyllum pinnatum (lam.) and Polyalthia longifolia (Sonn.) . Comparative study on the efficiency of the synthesized Copper oxide nanoparticles against each precursor has been studied. Different spectroscopic and microscopic characterization techniques such as UV- Visible spectrophotometer, X- ray powder diffraction (XRD), Fourier transformed infrared spectroscopy (FTIR), Scanning Electron Microscope (SEM) were performed to confirm the presence of copper oxide nanoparticles. UV –vis spectrophotometer results confirmed the existence of copper oxide nanoparticles using Copper chloride and Copper sulphate precursor showed absorption at 235nm and 575nm respectively. X- Ray Diffraction results showed crystalline structure of the particles with three peaks at (111), (200) & (220) which confirmed the presence of copper oxide nanoparticle for both the precursors. FTIR results supported the existence of several functional groups involved in capping, reducing, and stabilizing copper oxide nanoparticles. The SEM image showed that the copper oxide nanoparticles were spherical in shape and ranged in size from 40 to 90 nm. Further, the Anti-fungal and Anti-bacterial activity of the synthesized nanoparticle for both the copper chloride and copper sulphate precursor were studied. The Study shown maximum zone of inhibition at 100µg/ml as 18mm and 25mm respectively against Galactomyces geotrichum. As a result of the high biological potentials and powerful Antifungal activity, the green synthesized copper oxide nanoparticles can be exploited in phytopathology to combat plant infections.
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