激素刺激和间歇性冷暴露对多囊卵巢综合征形成机制的影响

I. Kuzmina
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摘要

介绍。多囊卵巢综合征(PCOS)是妇科内分泌学研究的热点问题之一。本研究旨在探讨不同激素暴露对PCOS大鼠卵巢形态的影响。材料和方法。为了研究,开发了三个有效的实验模型,并将各种激素药物引入动物体内。模型I-st为含雌激素药物皮下注射,剂量为3mg,持续25天。模型II-d为每日皮下注射3mg硫酸脱氢雄烯二醇导致多囊卵巢综合征。III模型- 5只未成熟大鼠在皮下注射多巴胺植物类似物3mg,持续25天,形成多囊卵巢综合征。将实验动物分为3组:1组为实验性PCOS诱导大鼠,在+23°C的环境中饲养(n=8); 2组为实验性PCOS背景下,在+ 4°C恒定光照条件下冷暴露(CE) 4小时,持续25天(n=8);3组为未患多囊卵巢综合征的健康大鼠(n = 8),饲养于+ 23℃恒温箱内。结果。1组大鼠卵巢组织学检查显示PCOS卵巢形态学改变。卵巢形态结构符合II型多囊卵巢综合征。从宏观上看,3组大鼠卵巢尺寸较对照组略有缩小。脂联素和瘦素水平作为PCOS模型实验卵巢功能组织的标志物。在研究CE对实验动物身体的影响时,确定了CE对组织细胞增生形成的影响,这是PCOS的特征标志。结论。研究表明,多囊卵巢综合征的形成以及卵巢的结构变化在很大程度上取决于激素调节障碍。
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Influence of hormonal stimulation and intermittent cold exposure on the mechanisms of formation of polycystic ovarian syndrome
Introduction. Polycystic ovary syndrome (PCOS) is one of the most pressing problems in gynecological endocrinology. The aim of the study was to elucidate the morphological changes in the ovaries of rats under various types of hormonal exposure in experimental modeling of PCOS. Materials and methods. To study three working experimental models were developed, associated with the introduction of various hormonal drugs into the body of an animal. Model I-st consisted of subcutaneous administration of an estrogen-containing drug at a dose of 3 mg for 25 days. Model II-d involved the development of PCOS by daily subcutaneous administration of 3 mg of dehydroandrostenediol sulfate. The III model - the formation of PCOS in 5 immature rats using dopamine phytoanalogues at a dose of 3 mg subcutaneously for 25 days. The animals were divided into 3 groups: group 1 - rats that were induced with experimental PCOS and kept in a vivarium at a temperature of +23°C (n=8), group 2 - animals that, against the background of experimental PCOS, were exposed to cold exposure (CE) for 4 hours in a chamber with a constant light regime and a temperature of + 4 °C for 25 days (n = 8); group 3 - intact control (n = 8) - healthy rats without PCOS, which were kept in a vivarium at a temperature of + 23°C. Results. Histological examination of the ovaries of group 1 rats revealed morphological changes in the ovaries characteristic of PCOS. The morphological structure of the ovaries corresponded to type II PCOS. In rats of group 3, macroscopically, the ovaries were slightly reduced in size compared to the control group. The levels of adiponectin and leptin as markers of the functional ovarian tissue of PCOS model experiments.When studying the effect of CE on the body of experimental animals, their effect on the formation of tissue cell hyperplasia as a characteristic sign of PCOS was determined. Conclusions. Studies have shown that the formation of PCOS, as well as structural changes in the ovaries, significantly depend on hormonal regulation disorders.
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