{"title":"盐酸克仑特罗和莱克多巴胺的等密度无溶剂流动相HPLC-PDA分析","authors":"Naoto Furusawa","doi":"10.1016/j.ijcas.2013.07.002","DOIUrl":null,"url":null,"abstract":"<div><p>This paper describes a reserved-phase HPLC method for detecting clenbuterol (CB) and ractopamine (RP) using an isocratic solvent-free mobile phase. The separations were performed a Kaseisorb<sup>®</sup> LC C1-300-5 (100 × 4.6 mm, 5 μm) with 0.05 mol/L octane sulfonic acid mobile phase and a photodiode array detector. The total run time was <5 min. The system suitability was well within the international acceptance criteria. The detection limits were 0.68 ng/mL for CB and 1.24 ng/mL for RP, respectively. A harmless method for simultaneous detecting CB and RP was developed and may be further applied to the quantification in foods.</p></div>","PeriodicalId":100693,"journal":{"name":"International Journal of Chemical and Analytical Science","volume":"4 3","pages":"Pages 169-173"},"PeriodicalIF":0.0000,"publicationDate":"2013-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.ijcas.2013.07.002","citationCount":"6","resultStr":"{\"title\":\"An isocratic solvent-free mobile phase HPLC-PDA analysis of clenbuterol and ractopamine\",\"authors\":\"Naoto Furusawa\",\"doi\":\"10.1016/j.ijcas.2013.07.002\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>This paper describes a reserved-phase HPLC method for detecting clenbuterol (CB) and ractopamine (RP) using an isocratic solvent-free mobile phase. The separations were performed a Kaseisorb<sup>®</sup> LC C1-300-5 (100 × 4.6 mm, 5 μm) with 0.05 mol/L octane sulfonic acid mobile phase and a photodiode array detector. The total run time was <5 min. The system suitability was well within the international acceptance criteria. The detection limits were 0.68 ng/mL for CB and 1.24 ng/mL for RP, respectively. A harmless method for simultaneous detecting CB and RP was developed and may be further applied to the quantification in foods.</p></div>\",\"PeriodicalId\":100693,\"journal\":{\"name\":\"International Journal of Chemical and Analytical Science\",\"volume\":\"4 3\",\"pages\":\"Pages 169-173\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2013-09-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/j.ijcas.2013.07.002\",\"citationCount\":\"6\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International Journal of Chemical and Analytical Science\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0976120913000284\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Chemical and Analytical Science","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0976120913000284","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
An isocratic solvent-free mobile phase HPLC-PDA analysis of clenbuterol and ractopamine
This paper describes a reserved-phase HPLC method for detecting clenbuterol (CB) and ractopamine (RP) using an isocratic solvent-free mobile phase. The separations were performed a Kaseisorb® LC C1-300-5 (100 × 4.6 mm, 5 μm) with 0.05 mol/L octane sulfonic acid mobile phase and a photodiode array detector. The total run time was <5 min. The system suitability was well within the international acceptance criteria. The detection limits were 0.68 ng/mL for CB and 1.24 ng/mL for RP, respectively. A harmless method for simultaneous detecting CB and RP was developed and may be further applied to the quantification in foods.
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