{"title":"载酪蛋白壳聚糖微颗粒的白果乙醇提取物对体外人前列腺癌细胞的细胞毒性诱导作用","authors":"Kanchana Amarnath, Jeevitha Dhanabal, Isha Agarwal, Srividya Seshadry","doi":"10.1016/j.bionut.2013.03.009","DOIUrl":null,"url":null,"abstract":"<div><p>The present exploration reports an extensive evaluation of <em>in vitro</em> anticancer efficacy of a novel aqueous ethanolic extract of <em>Acalypha indica</em><span> (ETAI) loaded chitosan-casein (CS-CT) microparticles<span><span> in a cancer cell line model. Spherically shaped ETAI loaded CS-CT (CS/CT/ETAI) microparticles were prepared by colloidal </span>coacervation technique in a completely aqueous environment with an entrapment efficiency and particle size of 85.30</span></span> <!-->±<!--> <!-->4.10 and 2<!--> <!-->±<!--> <!-->0.96<!--> <span>μm respectively. Cytotoxicity, as investigated on human prostate cancer cell line<span> (PC3) by MTT assay, revealed insignificant differences between free ETAI and CS/CT/ETAI microparticles treated cells in the first 24</span></span> <!-->h, while higher cytotoxicity was demonstrated by CS/CT/ETAI microparticles, following 72<!--> <span>h of incubation. Percentage of cytotoxicity also demonstrated by LDH assay, which shown the concentration dependent leakage of LDH from PC3 cells exposed to free ETAI and CS/CT/ETAI microparticles The use of significantly low concentration of </span><em>Acalypha indica</em> loaded with CS/CT is a much better approach in comparison to the use of free ETAI for cancer treatment in future.</p></div>","PeriodicalId":100182,"journal":{"name":"Biomedicine & Preventive Nutrition","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2014-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.bionut.2013.03.009","citationCount":"15","resultStr":"{\"title\":\"Cytotoxicity induction by ethanolic extract of Acalypha indica loaded casein-chitosan microparticles in human prostate cancer cell line in vitro\",\"authors\":\"Kanchana Amarnath, Jeevitha Dhanabal, Isha Agarwal, Srividya Seshadry\",\"doi\":\"10.1016/j.bionut.2013.03.009\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>The present exploration reports an extensive evaluation of <em>in vitro</em> anticancer efficacy of a novel aqueous ethanolic extract of <em>Acalypha indica</em><span> (ETAI) loaded chitosan-casein (CS-CT) microparticles<span><span> in a cancer cell line model. Spherically shaped ETAI loaded CS-CT (CS/CT/ETAI) microparticles were prepared by colloidal </span>coacervation technique in a completely aqueous environment with an entrapment efficiency and particle size of 85.30</span></span> <!-->±<!--> <!-->4.10 and 2<!--> <!-->±<!--> <!-->0.96<!--> <span>μm respectively. Cytotoxicity, as investigated on human prostate cancer cell line<span> (PC3) by MTT assay, revealed insignificant differences between free ETAI and CS/CT/ETAI microparticles treated cells in the first 24</span></span> <!-->h, while higher cytotoxicity was demonstrated by CS/CT/ETAI microparticles, following 72<!--> <span>h of incubation. Percentage of cytotoxicity also demonstrated by LDH assay, which shown the concentration dependent leakage of LDH from PC3 cells exposed to free ETAI and CS/CT/ETAI microparticles The use of significantly low concentration of </span><em>Acalypha indica</em> loaded with CS/CT is a much better approach in comparison to the use of free ETAI for cancer treatment in future.</p></div>\",\"PeriodicalId\":100182,\"journal\":{\"name\":\"Biomedicine & Preventive Nutrition\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2014-07-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/j.bionut.2013.03.009\",\"citationCount\":\"15\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biomedicine & Preventive Nutrition\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2210523913000214\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biomedicine & Preventive Nutrition","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2210523913000214","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Cytotoxicity induction by ethanolic extract of Acalypha indica loaded casein-chitosan microparticles in human prostate cancer cell line in vitro
The present exploration reports an extensive evaluation of in vitro anticancer efficacy of a novel aqueous ethanolic extract of Acalypha indica (ETAI) loaded chitosan-casein (CS-CT) microparticles in a cancer cell line model. Spherically shaped ETAI loaded CS-CT (CS/CT/ETAI) microparticles were prepared by colloidal coacervation technique in a completely aqueous environment with an entrapment efficiency and particle size of 85.30 ± 4.10 and 2 ± 0.96 μm respectively. Cytotoxicity, as investigated on human prostate cancer cell line (PC3) by MTT assay, revealed insignificant differences between free ETAI and CS/CT/ETAI microparticles treated cells in the first 24 h, while higher cytotoxicity was demonstrated by CS/CT/ETAI microparticles, following 72 h of incubation. Percentage of cytotoxicity also demonstrated by LDH assay, which shown the concentration dependent leakage of LDH from PC3 cells exposed to free ETAI and CS/CT/ETAI microparticles The use of significantly low concentration of Acalypha indica loaded with CS/CT is a much better approach in comparison to the use of free ETAI for cancer treatment in future.
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