越南部分葫芦科植物种子油中甘油三酯和共轭脂肪酸组成分析

N. Anh, V. Anh
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摘要

共轭脂肪酸被认为是制药工业的潜在来源,具有有益的生物活性,包括抗癌作用,抗糖尿病,抗肥胖和抗炎特性。本文对五种植物(Momordica Cochinchinensis, Lour.)种子油的甘油三酯和脂肪酸的特性进行了研究。采用紫外光谱、傅里叶红外光谱和反相高效液相色谱技术对越南产的春、苦瓜(MomordicaMomordica charantia)、海苔(Thladiantha nudiflora Hemsl)和瓜蒌(trichosans cucumerina var. anguina)进行了分析。采用DAD检测器与MS检测器串联的反相高效液相色谱法测定种子油中甘油三酯的组成。以α-骨脂酸(C18:39E,11E,13Z)为底酸,以β-骨脂酸为次共轭酸为共轭十八碳三烯酸的丰富来源。在南洋蓟种子中,α-骨酸和癸酸(C18:39Z,11E,13Z)的合成比例大致相等。以黄瓜籽油为原料,合成了以石榴酸为主要共轭脂肪酸的化合物。此外,还测定了这些种子油的出油率和折射率等油参数。
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Analysis of Triacylglyceride and Conjugated Fatty Acid Compositions in Seed Oils of some Plants of Cucurbitaceae Family in Vietnam
Conjugated fatty acids were known as potential sources for the pharmaceutical industry with beneficial bioactivities, including anti-cancer effects, anti-diabetes, anti-obesity, and anti-inflammatory properties. In the present work, characteristics of triglycerides and fatty acids of the seed oils of five plants (Momordica Cochinchinensis (Lour.) Spreng, MomordicaMomordica charantia, Thladiantha nudiflora Hemsl, and Trichosanthes cucumerina var. anguina) in Vietnam were analyzed by UV and FTIR spectroscopy, and reversed phase high performance liquid chromatography. Triacylglycerides compositions of the  seed oils were determined using RP-HPLC with DAD detector connected with MS detector in series. The seed oils of M. Cochinchinensis, M. subangulata, and M. charantia are rich sources of conjugated octadecatrienoic acid with α-eleostearic acid (C18:39E,11E,13Z) as the base acid and β-eleostearic as the minor conjugated acid. In the seed of Thladiantha nudiflora (both α-eleostearic and punic acid (C18:39Z,11E,13Z)  were synthesized with approximately equal proportions. In T. cucumerina var. anguina seed oil, punicic acid was synthesized as the main conjugated fatty acid. In addition, some oil parameters, such as oil yield and refractive index of these seed oils, were determined.
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