河岸土壤AK3链霉菌制备银纳米颗粒的抗菌性能研究

Arun Kumar Kulshrestha, P. Patel
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摘要

摘要:土壤样品取自印度古吉拉特邦苏拉特的Tapi河重金属污染场地,经连续稀释后分散在放线菌分离培养基上。分离株在100 ml淀粉酪蛋白肉汤中,300℃,摇培养72小时。将无细胞滤液加入到1 mM硝酸银的终溶液中,然后在2500℃下干燥,使用分光光度计定量银纳米颗粒,从Malvern获得尺寸分布和zeta电位数据,并在PCR混合物中扩增16S rRNA基因。在atacamensis菌株AK3滤液中加入硝酸银后,还原剂使肉汤的颜色由黄色变为浅棕色。在420 nm处吸光度最高,0.25的多分散性指数低于团聚阈值。透射电镜显示,它们的形状为球形至椭球状,尺寸为20 nm。NJ序列比对结果显示,该菌株与S. atacamensis C60的相似性为99.42%。35 μg/ml AgNPs时,该菌株对表皮葡萄球菌、鲍曼单胞杆菌、淋病奈索菌和单核增生乳杆菌的抑制区分别为18±1 mm、19±1 mm、20±1 mm和14±1 mm,证明了该菌株合成AgNPs的有效性。
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Investigating the Antibacterial Properties of Silver Nanoparticles Acquired using Streptomyces strain AK3 from Riverbank Soil
ABSTRACT: The soil sample was acquired from a heavily metal polluted site on the Tapi River in Surat, Gujarat, India, diluted serially, and dispersed over an actinomycetes isolation medium. Isolates were cultured in 100 ml of starch-casein broth at 300 C for 72 hours in an incubator with shaking. The cell-free filtrate was added to a final solution of 1 mM silver nitrate, which was then dried at 2500 C. Using a spectrophotometer, silver nanoparticles were quantified, data on size distribution and zeta potential were acquired from Malvern, and the 16S rRNA gene was amplified in a PCR mixture. As a result of the addition of silver nitrate to the S. atacamensis strain AK3 filtrate, the reducers altered the broth's color from yellow to light brown. The highest absorbance was measured at 420 nm, and the 0.25 polydispersity index was below the agglomeration threshold. The TEM indicated their spherical to ellipsoidal shape and 20 nm size. The NJ approach to sequence alignment revealed that the strain was 99.42% similar to S. atacamensis C60. Zones of inhibition of S. epidermidis, A. baumanni, N. gonorrhoeae, and L. monocytogenes were found to be 18±1 mm, 19±1 mm, 20±1 mm, and 14±1 mm respectively, at 35 μg/ml AgNPs, proving the efficiency of AgNPs synthesized by the strain.
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