无花果树组分抗利什曼原虫活性研究

A. Bargougui , P. Champy , S. Triki , C. Bories , P. Le Pape , P.M. Loiseau
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引用次数: 6

摘要

无花果树的枝和果实在传统医学中用于治疗脓肿和皮肤炎症。因此,评估抗利什曼原虫活性是否与皮肤愈合有关是很有趣的。本文报道了榕树枝和果实提取物的抗利什曼原虫活性。枝蔓果乙酸乙酯提取物对大利什曼原虫的IC50值为53.9 μg/mL,而乙酸乙酯果提取物、枝蔓果乙酸乙酯提取物和枝蔓甲醇提取物对多诺瓦利什曼原虫的IC50值分别为70.3、70.5和45.2 μg/mL。该组分对布氏锥虫的活性较差。最后,对无花果果实进行生物引导分离得到的预纯化组分对巨噬细胞内多诺瓦利什曼原虫的IC50为9.3 μg/mL。选择性指数定义为CC50/IC50大于10。综上所述,与乙酸乙酯果实提取物相比,生物引导分离可使其抗利什曼原虫活性提高约10倍。这种活性值得进一步研究,以确定产生抗利什曼病作用的化合物。
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Antileishmanial activity of Opuntia ficus-indica fractions

Cladodes and fruits of Opuntia ficus-indica are used in traditional medicine for the treatment of abscess and skin inflammation. It was therefore interesting to assess whether an antileishmanial activity could be associated to skin healing. This study reports on the antileishmanial activity of Opuntia ficus-indica extracts from cladodes and fruits. Ethyl acetate extract from cladodes only exhibited an activity against Leishmania major with an IC50 value of 53.9 μg/mL, but ethyl acetate fruit extract, ethyl acetate cladode extract and methanol cladode extract were active also against Leishmania donovani with IC50 values at 70.3, 70.5 and 45.2 μg/mL, respectively. A poor activity of the fractions was monitored against Trypanosoma brucei brucei. Finally, a bioguided fractionation of fruits of Opuntia ficus-indica led to a pre-purified fraction that exhibited an IC50 of 9.3 μg/mL against Leishmania donovani intramacrophage amastigotes. The selectivity index defined as CC50/IC50 was higher than 10. In conclusion, the bioguided fractionation allowed to enhance the antileishmanial activity about ten-fold comparatively to those of the ethyl acetate fruit extract. Such an activity is worth of further investigations to identify the compounds responsible for the antileishmanial effect.

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