Rat Bioassay of Combinations of Diuretics*

A bioassay procedure was used to determine whether or not combinations of acetazolamide sodium with selected diuretic agents would yield more effective responses than those induced by the individual components of a given combination. The diuretics chosen for combination with acetazolamide sodium were: caffeine, mercurophylline, potassium acetate, potassium bicarbonate, potassium citrate, potassium nitrate, sodium acetate, theobromine, and theophylline. The best combinations of relatively weak diuretics were obtained with the acetazolamide sodium + potassium bicarbonate and acetazolamide sodium + potassium acetate series. Both the acetazolamide sodium + caffeine and acetazolamide sodium + potassium nitrate combinations warrant more critical evaluation.