{"title":"联合用药大鼠血液中阿米替林的高效液相色谱检测方法","authors":"Rabie Saad Farag , Mahmoud Zaki Darwish , Wael Mohamed Fathy , Hamdi Ahmed Hammad","doi":"10.1016/j.ijcas.2013.06.001","DOIUrl":null,"url":null,"abstract":"<div><p>A rapid, sensitive and specific method based on high performance liquid chromatography (HPLC) has been developed for the simultaneous determination of amitriptyline hydrochloride and Sildenafil citrate in the blood of rats. Sample preparation involved liquid–liquid extraction with n-hexane after alkalified with 0.5 M NaOH. Chromatographic separation was performed on XDB C-18 column with particle size of 5 μm (150 × 4.6 mm) with mobile phase acetonitrile and 20 mM KH<sub>2</sub>PO<sub>4</sub> buffer, pH = 3 using flow rate 1.5 ml/min. Calibration curves were linear within the ranges of 0.25–50 and 0.1–50 μg/ml for amitriptyline hydrochloride and Sildenafil citrate, respectively. This method was successfully applied to the pharmacokinetic study in rats after oral administration of amitriptyline hydrochloride and Sildenafil citrate for 24 h.</p></div>","PeriodicalId":100693,"journal":{"name":"International Journal of Chemical and Analytical Science","volume":"4 2","pages":"Pages 120-124"},"PeriodicalIF":0.0000,"publicationDate":"2013-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.ijcas.2013.06.001","citationCount":"14","resultStr":"{\"title\":\"New HPLC method to detect amitriptyline in the blood of rats on combination treatment\",\"authors\":\"Rabie Saad Farag , Mahmoud Zaki Darwish , Wael Mohamed Fathy , Hamdi Ahmed Hammad\",\"doi\":\"10.1016/j.ijcas.2013.06.001\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>A rapid, sensitive and specific method based on high performance liquid chromatography (HPLC) has been developed for the simultaneous determination of amitriptyline hydrochloride and Sildenafil citrate in the blood of rats. Sample preparation involved liquid–liquid extraction with n-hexane after alkalified with 0.5 M NaOH. Chromatographic separation was performed on XDB C-18 column with particle size of 5 μm (150 × 4.6 mm) with mobile phase acetonitrile and 20 mM KH<sub>2</sub>PO<sub>4</sub> buffer, pH = 3 using flow rate 1.5 ml/min. Calibration curves were linear within the ranges of 0.25–50 and 0.1–50 μg/ml for amitriptyline hydrochloride and Sildenafil citrate, respectively. This method was successfully applied to the pharmacokinetic study in rats after oral administration of amitriptyline hydrochloride and Sildenafil citrate for 24 h.</p></div>\",\"PeriodicalId\":100693,\"journal\":{\"name\":\"International Journal of Chemical and Analytical Science\",\"volume\":\"4 2\",\"pages\":\"Pages 120-124\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2013-06-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/j.ijcas.2013.06.001\",\"citationCount\":\"14\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International Journal of Chemical and Analytical Science\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0976120913000223\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Chemical and Analytical Science","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0976120913000223","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 14
摘要
建立了快速、灵敏、特异的高效液相色谱法同时测定大鼠血液中盐酸阿米替林和柠檬酸西地那非的方法。样品制备采用0.5 M NaOH碱化后，正己烷液液萃取。色谱柱为XDB C-18，色谱柱粒径为5 μm (150 × 4.6 mm)，流动相为乙腈和20 mm KH2PO4缓冲液，pH = 3，流速为1.5 ml/min。盐酸阿米替林和柠檬酸西地那非在0.25 ~ 50和0.1 ~ 50 μg/ml范围内呈线性关系。本方法成功应用于大鼠口服盐酸阿米替林和枸橼酸西地那非24 h后的药代动力学研究。
New HPLC method to detect amitriptyline in the blood of rats on combination treatment
A rapid, sensitive and specific method based on high performance liquid chromatography (HPLC) has been developed for the simultaneous determination of amitriptyline hydrochloride and Sildenafil citrate in the blood of rats. Sample preparation involved liquid–liquid extraction with n-hexane after alkalified with 0.5 M NaOH. Chromatographic separation was performed on XDB C-18 column with particle size of 5 μm (150 × 4.6 mm) with mobile phase acetonitrile and 20 mM KH2PO4 buffer, pH = 3 using flow rate 1.5 ml/min. Calibration curves were linear within the ranges of 0.25–50 and 0.1–50 μg/ml for amitriptyline hydrochloride and Sildenafil citrate, respectively. This method was successfully applied to the pharmacokinetic study in rats after oral administration of amitriptyline hydrochloride and Sildenafil citrate for 24 h.
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