Miceller chromatographic method for the separation of heavy metal ions and spectrophotometric estimation of UO22+ on bismuth silicate layer

Context

Micellar thin layer chromatography (MLC) used for separation of various heavy metal ions from various environmental samples. MLC successfully applied for semiquanitative and spectrometry identification of metal ions.

Objective

Utilization of MLC in separation, semiquanitative determination and Chromatospectrometric method for estimation of ${\text{UO}}_2^{2+}$ on bismuth silicate layer, using Tween-80.

Method

Ascending development of the plates performed with aq. micellar, hydro-organic and water-organic-surfactant as mobile phase. Separation carried out on thin layer of bismuth silicate and layer developed with non-ionic surfactant Tween-80.

Results and conclusions

Acetone was found to be most effective additive at 10% concentration with 5% Tween-80. This method successfully applied to identification and separation of Cd₂, Hg₂ and Co₂ from river water and industrial wastewater. Semiquantitative as well as spectrometry techniques was used for quantitative analysis of ${\text{UO}}_2^{2+}$ in river water with preliminary separation from Fe₃ and Cu₂. Maximal recovery of 90% observed. This TLC method is rapid, with development times averaging 2–5 min.