毛霉菌的菌落年龄会改变挥发性有机化合物的分布以及抑制豆类植物中硬皮病菌的能力。

IF 1.8 3区 农林科学 Q2 PLANT SCIENCES Plant Pathology Journal Pub Date : 2023-02-01 DOI:10.5423/PPJ.OA.08.2022.0106
Lincon Rafael da Silva, Leonardo Luís de Barros Rodrigues, Amanda Silva Botelho, Bruna Sartório de Castro, Paulo Henrique Pereira Costa Muniz, Maria Carolina Blassioli Moraes, Sueli Corrêa Marques de Mello
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摘要

蚕豆(Phaseolus vulgaris L.)是人类粮食生产中最重要的作物之一。由硬粒菌(Sclerotinia sclerotiorum)引起的白霉等病害的发生会限制这种豆科植物的产量。使用毛霉菌已成为抑制这种病害的重要策略。本研究旨在评估 Trichoderma azevedoi CEN1241 在五个不同生长期释放的挥发性有机化合物(VOCs)对蚕豆白霉严重程度的影响。体外试验采用双板和分板法进行,体内试验则是将硬毛菌的菌丝体暴露于 T. azevedoi CEN1241 的挥发性有机化合物中,然后接种到蚕豆植株中。通过气相色谱-质谱联用技术进行的化学分析检测出 T. azevedoi CEN1241 产生的 37 种挥发性有机化合物,涵盖六大类化学物质。T. azevedoi CEN1241 产生的挥发性有机化合物随菌落年龄的不同而变化,并被证明与生物控制剂抑制硬核菌的能力有关。T. azevedoi CEN1241 的挥发性有机化合物能在体外缩小豆角硬皮病病斑的大小,并在温室中减轻病害的严重程度。这项研究以一种更实用的方式证明,通过毛霉产生的挥发性化合物进行抗菌的机制可以补充其他机制,如寄生和竞争机制,从而有助于更有效地控制豆科植物的白霉病。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Colony Age of Trichoderma azevedoi Alters the Profile of Volatile Organic Compounds and Ability to Suppress Sclerotinia sclerotiorum in Bean Plants.

Common bean (Phaseolus vulgaris L.) is one of the most important crops in human food production. The occurrence of diseases, such as white mold, caused by Sclerotinia sclerotiorum can limit the production of this legume. The use of Trichoderma has become an important strategy in the suppression of this disease. The aim of the present study was to evaluate the effect of volatile organic compounds (VOCs) emitted by Trichoderma azevedoi CEN1241 in five different growth periods on the severity of white mold in common bean. The in vitro assays were carried out in double-plate and split-plate, and the in vivo assays, through the exposure of the mycelia of S. sclerotiorum to the VOCs of T. azevedoi CEN1241 and subsequent inoculation in bean plants. Chemical analysis by gas chromatography coupled to mass spectrometry detected 37 VOCs produced by T. azevedoi CEN1241, covering six major chemical classes. The profile of VOCs produced by T. azevedoi CEN1241 varied according to colony age and was shown to be related to the ability of the biocontrol agent to suppress S. sclerotiorum. T. azevedoi CEN1241 VOCs reduced the size of S. sclerotiorum lesions on bean fragments in vitro and reduced disease severity in a greenhouse. This study demonstrated in a more applied way that the mechanism of antibiosis through the production of volatile compounds exerted by Trichoderma can complement other mechanisms, such as parasitism and competition, thus contributing to a better efficiency in the control of white mold in bean plants.

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来源期刊
Plant Pathology Journal
Plant Pathology Journal 生物-植物科学
CiteScore
4.90
自引率
4.30%
发文量
71
审稿时长
12 months
期刊介绍: Information not localized
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