利用磁感应技术对工程骨骼肌功能进行高通量实时监测。

IF 6.7 1区 工程技术 Q1 CELL & TISSUE ENGINEERING Journal of Tissue Engineering Pub Date : 2022-09-02 eCollection Date: 2022-01-01 DOI:10.1177/20417314221122127
Alec St Smith, Shawn M Luttrell, Jean-Baptiste Dupont, Kevin Gray, Daniel Lih, Jacob W Fleming, Nathan J Cunningham, Sofia Jepson, Jennifer Hesson, Julie Mathieu, Lisa Maves, Bonnie J Berry, Elliot C Fisher, Nathan J Sniadecki, Nicholas A Geisse, David L Mack
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引用次数: 0

摘要

工程肌肉组织是研究骨骼肌组织级收缩特性的有力工具。然而,标准分析方法的通量局限性限制了它们在纵向研究、高通量药物筛选和疾病建模中的应用。在这里,我们提出了一种将三维工程骨骼肌与磁感应系统集成的方法,以促进对发育中的收缩动力学进行非侵入式纵向分析。利用这一平台,我们展示了诱导多能干细胞和原始来源的工程骨骼肌组织在培养过程中随着时间的推移在收缩输出方面的改善。我们展示了如何利用磁感应收缩力来同时评估多个组织在不同剂量的已知骨骼肌肌力促进剂作用下的情况,以及正常和萎缩性肌肉细胞中健康与疾病功能特征的分层。基于这些数据,该组合培养系统和磁性收缩力平台极大地拓宽了三维工程骨骼肌组织的潜力,从而影响新型疗法从实验室到临床的转化。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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High-throughput, real-time monitoring of engineered skeletal muscle function using magnetic sensing.

Engineered muscle tissues represent powerful tools for examining tissue level contractile properties of skeletal muscle. However, limitations in the throughput associated with standard analysis methods limit their utility for longitudinal study, high throughput drug screens, and disease modeling. Here we present a method for integrating 3D engineered skeletal muscles with a magnetic sensing system to facilitate non-invasive, longitudinal analysis of developing contraction kinetics. Using this platform, we show that engineered skeletal muscle tissues derived from both induced pluripotent stem cell and primary sources undergo improvements in contractile output over time in culture. We demonstrate how magnetic sensing of contractility can be employed for simultaneous assessment of multiple tissues subjected to different doses of known skeletal muscle inotropes as well as the stratification of healthy versus diseased functional profiles in normal and dystrophic muscle cells. Based on these data, this combined culture system and magnet-based contractility platform greatly broadens the potential for 3D engineered skeletal muscle tissues to impact the translation of novel therapies from the lab to the clinic.

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来源期刊
Journal of Tissue Engineering
Journal of Tissue Engineering Engineering-Biomedical Engineering
CiteScore
11.60
自引率
4.90%
发文量
52
审稿时长
12 weeks
期刊介绍: The Journal of Tissue Engineering (JTE) is a peer-reviewed, open-access journal dedicated to scientific research in the field of tissue engineering and its clinical applications. Our journal encompasses a wide range of interests, from the fundamental aspects of stem cells and progenitor cells, including their expansion to viable numbers, to an in-depth understanding of their differentiation processes. Join us in exploring the latest advancements in tissue engineering and its clinical translation.
期刊最新文献
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