M D Alonso-Díaz de Durana, E Pérez-Fernández, M Villalba, L Martín-Pedraza, M Fernández-Rivas
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Mutual immunoblotting inhibition of olive and cypress extracts and inhibition of cypress extract immunoblotting with olive allergens were performed. Multiple correspondence analysis and hierarchical cluster classifications were conducted to analyze the relationships between the clinical presentation of C+O (symptoms, seasonality) and allergen profile.</p><p><strong>Results: </strong>C+O patients were clustered in 4 phenotypes. The most frequent one (58.4%) was rhinoconjunctivitis in winter (February) and spring (May), with asthma in 38% of patients. Ole e 1 and Cup s 1 were the major allergens. Proteins homologous to Ole e 1, Ole e 9, and Ole e 11 in cypress pollen were identified and shown to inhibit IgE binding to cypress extract.</p><p><strong>Conclusions: </strong>The exclusive C+O results from cosensitization to Cup s 1 and Ole e 1 and cross-reactivity due to previously unreported Ole e 1-like, Ole e 9-like, and Ole e 11-like allergens in cypress pollen. 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引用次数: 0
摘要
背景和目标:背景:柏树花粉和橄榄花粉是地中海地区最常见的致敏物质。一些患者表现出双重过敏,但迄今为止还没有很好的记录。目的:确定涉及双重过敏的过敏原:确定涉及柏树和橄榄双重过敏(C+O)的过敏原,并研究表型与过敏原致敏之间的关系:方法:筛选出 C+O 患者。方法:选择 C+O 患者,以对橄榄或扁柏单敏的患者为参照。测定对橄榄和柏树的整体提取物和纯化过敏原的特异性 IgE。分别使用橄榄多克隆抗体和患者血清进行免疫印迹,分析 IgG 和 IgE 的结合情况。进行了橄榄和柏树提取物的相互免疫印迹抑制以及柏树提取物与橄榄过敏原的免疫印迹抑制。对 C+O 的临床表现(症状、季节性)和过敏原谱之间的关系进行了多重对应分析和分层聚类分析:结果:C+O 患者分为 4 种表型。最常见的表型(58.4%)是冬季(2 月)和春季(5 月)的鼻结膜炎,38%的患者患有哮喘。Ole e 1 和 Cup s 1 是主要的过敏原。发现了与柏树花粉中的 Ole e 1、Ole e 9 和 Ole e 11 同源的蛋白质,并证明它们能抑制 IgE 与柏树提取物的结合:结论:柏树花粉中的类似 Ole e 1、类似 Ole e 9 和类似 Ole e 11 的过敏原导致了对 Cup s 1 和 Ole e 1 的共敏性和交叉反应,从而产生了独有的 C+O 症状。我们的研究结果指出了冬季和/或春季或常年性鼻结膜炎伴有或不伴有哮喘的 4 种临床表型。
Allergy to Cypress and Olive Pollen: Clinical Phenotypes and Allergen Recognition.
Background and objectives: Background: Cypress and olive pollen are the most prevalent sensitizers in the Mediterranean area. Some patients exhibit dual sensitization, which has not been well documented to date. Objective: To identify the allergens involved in dual cypress and olive allergy (C+O) and to study the relationship between phenotype and allergen sensitization.
Methods: C+O patients were selected. Those monosensitized to olive or cypress were used as a reference. Specific IgE to whole extracts and purified allergens from olive and cypress was determined. Immunoblotting was performed to analyze IgG and IgE binding using olive polyclonal antibodies and patients' sera, respectively. Mutual immunoblotting inhibition of olive and cypress extracts and inhibition of cypress extract immunoblotting with olive allergens were performed. Multiple correspondence analysis and hierarchical cluster classifications were conducted to analyze the relationships between the clinical presentation of C+O (symptoms, seasonality) and allergen profile.
Results: C+O patients were clustered in 4 phenotypes. The most frequent one (58.4%) was rhinoconjunctivitis in winter (February) and spring (May), with asthma in 38% of patients. Ole e 1 and Cup s 1 were the major allergens. Proteins homologous to Ole e 1, Ole e 9, and Ole e 11 in cypress pollen were identified and shown to inhibit IgE binding to cypress extract.
Conclusions: The exclusive C+O results from cosensitization to Cup s 1 and Ole e 1 and cross-reactivity due to previously unreported Ole e 1-like, Ole e 9-like, and Ole e 11-like allergens in cypress pollen. Our findings point to 4 clinical phenotypes of winter and/or spring or perennial rhinoconjunctivitis with and without asthma.
期刊介绍:
The Journal of Investigational Allergology and Clinical Immunology (J Investig Allergol Clin Immunol) provides an attractive and very active forum for basic and clinical research in allergology and clinical immunology.Journal of Investigational Allergology and Clinical Immunology publishes original works, reviews, short communications and opinions.