绿色和化学合成氧化锌纳米颗粒对柑桔黑腐病病原菌柑橘交替病菌的抑菌活性研究。

IF 1.8 3区 农林科学 Q2 PLANT SCIENCES Plant Pathology Journal Pub Date : 2023-06-01 DOI:10.5423/PPJ.OA.02.2023.0035
Hazem S Elshafie, Ali Osman, Mahmoud M El-Saber, Ippolito Camele, Entsar Abbas
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摘要

柑桔黑腐病是由柑桔互交菌引起的柑桔严重病害。本研究旨在通过化学和绿色两种方法合成氧化锌纳米颗粒(ZnO-NPs),并研究其对柑橘的抗真菌活性。化学法和绿色法合成的ZnO-NPs的透射电镜尺寸分别为88 nm和65 nm。将制备的ZnO-NPs以不同浓度(500、1000和2000µg/ml)在采后处理脐橙果实,验证其对柑橘蚜的防治效果。体外实验结果表明,在浓度为2000µg/ml时,绿色ZnO-NPs能够抑制约61%的真菌生长,其次是化学ZnO-NPs能够抑制52%的真菌生长。此外,用绿色ZnO-NPs处理的柑桔体外扫描电镜显示分生孢子肿胀和变形。结果还表明,在柑桔人工侵染后采后,原位使用浓度为2000µg/ml的化学ZnO-NPs和绿色ZnO-NPs,贮藏20 d后,柑桔病的严重程度分别降低到6.92%和9.23%,而阳性对照(未处理的果实)的严重程度为23.84%。我们的研究结果可能有助于开发一种自然、有效、环保的方法来根除有害的植物病原真菌。
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Antifungal Activity of Green and Chemically Synthesized ZnO Nanoparticles against Alternaria citri, the Causal Agent Citrus Black Rot.

Citrus black rot is a serious disease of citrus plants caused by Alternaria citri. The current study aimed to synthesize zinc oxide nanoparticles (ZnO-NPs) by chemically or green method and investigate their antifungal activity against A. citri. The sizes of synthesized as measured by transmission electron microscope of ZnO-NPs were 88 and 65 nm for chemical and green methods, respectively. The studied prepared ZnO-NPs were applied, in vitro and in situ, at different concentrations (500, 1,000, and 2,000 µg/ml) in post-harvest treatment on navel orange fruits to verify the possible control effect against A. citri. Results of in vitro assay demonstrated that, at concentration 2,000 µg/ml, the green ZnO-NPs was able to inhibit about 61% of the fungal growth followed by 52% of chemical ZnO-NPs. In addition, scanning electron microscopy of A. citri treated in vitro with green ZnO-NPs showed swelling and deformation of conidia. Results showed also that, using a chemically and green ZnO-NPs at 2,000 µg/ml in situ in post-harvest treatment of orange, artificially-infected with A. citri, has reduced the disease severity to 6.92% and 9.23%, respectively, compared to 23.84% of positive control (non-treated fruits) after 20 days of storage. The out findings of this study may contribute to the development of a natural, effective, and eco-friendly strategy for eradicating harmful phytopathogenic fungi.

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来源期刊
Plant Pathology Journal
Plant Pathology Journal 生物-植物科学
CiteScore
4.90
自引率
4.30%
发文量
71
审稿时长
12 months
期刊介绍: Information not localized
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