Effect of sphingosine and inoculum concentrations on Staphylococcus aureus and Staphylococcus epidermidis biofilms.

IF 2.6 3区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Biofouling Pub Date : 2023-05-01 DOI:10.1080/08927014.2023.2236584

Minyoung Kim, Jada S McMahon, Julianna M Nerone, Luciana H Skinner, Guy K German, Cláudia N H Marques

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Abstract

Atopic dermatitis (AD) is a chronic inflammatory skin disease where patients are more susceptible to infection and inflammation. The most salient symptoms of atopic dermatitis (AD) are skin dysbiosis and ceramide deficiency. Here, the effect of AD conditions on S. aureus resilience was investigated. S. aureus and S. epidermidis biofilms were co-inoculated at healthy and AD bacterial ratios and exposed to various sphingosine dosing regimens. In both healthy (S. epidermidis dominant) and AD (S. aureus dominant) conditions the viability of the non-dominant bacterial species was affected. Quorum sensing (QS)-impaired S. aureus was overall more susceptible to sphingosine. Despite the general resilience of QS-intact S. aureus against sphingosine, modulation of S. epidermidis (healthy ratio) and sphingosine (healthy Sph) led to a lack of recovery from its initial killing. Overall, it was found that when in biofilms, S. epidermidis increases S. aureus resilience to sphingosine, possibly enhancing the pathogen's recalcitrance in AD skin.

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鞘氨醇和接种量对金黄色葡萄球菌和表皮葡萄球菌生物膜的影响。

特应性皮炎(AD)是一种慢性炎症性皮肤病，患者更容易感染和炎症。特应性皮炎(AD)最显著的症状是皮肤生态失调和神经酰胺缺乏。本文研究了AD条件对金黄色葡萄球菌恢复力的影响。金黄色葡萄球菌和表皮葡萄球菌生物膜以健康菌和AD菌的比例共接种，并暴露于不同的鞘氨醇剂量方案。在健康(表皮葡萄球菌优势)和AD(金黄色葡萄球菌优势)条件下，非优势细菌物种的生存能力都受到影响。群体感应(Quorum sensing, QS)受损的金黄色葡萄球菌总体上对鞘氨醇更敏感。尽管qs完整的金黄色葡萄球菌对鞘氨醇具有普遍的恢复力，但表皮葡萄球菌(健康比率)和鞘氨醇(健康Sph)的调节导致其最初被杀后缺乏恢复。总的来说，我们发现表皮葡萄球菌在生物膜中增加了金黄色葡萄球菌对鞘氨醇的抵抗力，可能增强了病原菌对AD皮肤的抵抗力。

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来源期刊

Biofouling 生物-海洋与淡水生物学

CiteScore

5.00

自引率

7.40%

发文量

审稿时长

1.7 months

期刊介绍： Biofouling is an international, peer-reviewed, multi-discliplinary journal which publishes original articles and mini-reviews and provides a forum for publication of pure and applied work on protein, microbial, fungal, plant and animal fouling and its control, as well as studies of all kinds on biofilms and bioadhesion. Papers may be based on studies relating to characterisation, attachment, growth and control on any natural (living) or man-made surface in the freshwater, marine or aerial environments, including fouling, biofilms and bioadhesion in the medical, dental, and industrial context. Specific areas of interest include antifouling technologies and coatings including transmission of invasive species, antimicrobial agents, biological interfaces, biomaterials, microbiologically influenced corrosion, membrane biofouling, food industry biofilms, biofilm based diseases and indwelling biomedical devices as substrata for fouling and biofilm growth, including papers based on clinically-relevant work using models that mimic the realistic environment in which they are intended to be used.