Study on adaptive responses of cells A549 and 2BS induced by low does irradiation

Abstract

Objective\ To investigate whether or not adaptive response of the 2BS and A549 cells induced by low dose irradiation.Moreover,to analyze the relationship between the adaptive response and cells cycle regulation. Methods\ 1.The dose survival curves of A549 and 2BS cells were figured by colony formation and typan blue cell staining.2.Flow cytometry was used to analyze the cell cycle after the different doses irradiation. Results\ 1. The adaptive response induced in 2BS cells by low dose,while it was not induced in A549 cells.2.G 2 phase arrest of 2BS cells after 75mGy X ray plus 4Gy 60 Co γ ray irradiation appeared in 30 minutes,and cell cycle recovered in 24 hours. Conclusion\ The adaptive response of 2BS cell could be induced by low dose irradiation,and there was close relationship between the adaptive responses and the regulation of cell cycle.