{"title":"Cellular targeting and subcellular distribution of HER-2 targeting boron liposomes","authors":"Zheng Shu, W. Qi-chun, Shengru. Li, E. Kullberg, L. Gedda","doi":"10.3760/CMA.J.ISSN.0254-5098.2005.04.004","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.0254-5098.2005.04.004","url":null,"abstract":"目的分析新制备的HER-2靶向硼脂质体在细胞靶向、滞留及细胞内分布等方面的特性,探讨该脂质体作为靶向给药系统用于硼中子俘获治疗研究的可能性.方法分别从靶向剂Trastuzumab、脂质体和装载的WSA 3个方面观察SK-BR-3细胞对125I-Trastuzumab-WSA-3H-脂质体的摄取与滞留.Trastuzumab标记了125I用伽玛计数器检测;脂质体标记了3H用液闪计数器检测;WSA用感应耦合等离子体-质谱仪检测.激光共聚焦显微镜观察WSA在细胞内的分布.结果最初8 h细胞对125I-Trastuzumab的摄取量快速增加,24 h后细胞内125I量反而有所下降.细胞对3H-脂质体的摄取随培养时间而增加,直到48 h也没有达到平台期.培养4、8及24 h,细胞含硼量分别达到55、78及132×10-6.细胞摄取125I-Trastuzumab-WSA-3H-脂质体后,在普通培养液中培养24和48 h,分别有90%、67%的WSA滞留在细胞内.激光共聚焦显微镜下显示大部分WSA位于细胞浆.结论细胞摄取量满意,WSA在细胞内滞留时间长,说明125I-Trastuzumab-WSA-3H-脂质体是一个很好的运载工具,有希望应用于硼中子俘获治疗研究.","PeriodicalId":374575,"journal":{"name":"Chinese Journal of Radiation Mediation and Protection","volume":"96 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2005-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122999874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2002-09-25DOI: 10.3760/CMA.J.ISSN.0254-5098.2002.05.009
Cui-lan Zhang
Objective To determine the damage of DNA in peripheral blood lymphocytes samples from resident exposure to radon in indoor air. Methods Single cell gel electrophoresis and micronucleus measure methods were used in the present study. Results The results of cell migration frequencies for residents in cave dwellings and normal houses were shown 1^68% and 1^43%,of calculation by residents were 57 1% and 54 3% respectively.The frequencies of micronucleus results for residents of cave dwellings and normal houses were 1 07‰ and 0^78‰ respectively. Conclusion The frequencies of DNA damage and the ratio of micronucleus for residents living in cave dwellings was shown some higher than that of residents living in normal houses, p values close to 0 05.;
{"title":"DNA damage in peripheral blood lymphocytes of residents with radon exposure in indoor","authors":"Cui-lan Zhang","doi":"10.3760/CMA.J.ISSN.0254-5098.2002.05.009","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.0254-5098.2002.05.009","url":null,"abstract":"Objective To determine the damage of DNA in peripheral blood lymphocytes samples from resident exposure to radon in indoor air. Methods Single cell gel electrophoresis and micronucleus measure methods were used in the present study. Results The results of cell migration frequencies for residents in cave dwellings and normal houses were shown 1^68% and 1^43%,of calculation by residents were 57 1% and 54 3% respectively.The frequencies of micronucleus results for residents of cave dwellings and normal houses were 1 07‰ and 0^78‰ respectively. Conclusion The frequencies of DNA damage and the ratio of micronucleus for residents living in cave dwellings was shown some higher than that of residents living in normal houses, p values close to 0 05.;","PeriodicalId":374575,"journal":{"name":"Chinese Journal of Radiation Mediation and Protection","volume":"8 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2002-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131227212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2001-03-25DOI: 10.3760/CMA.J.ISSN.0254-5098.2001.02.004
Xiaoling Wang, Jin Li
Objective To study dose|response relationship for chromosome translocation and its persistence measured in human lymphocytes exposed to 60 Co γ rays.Methods The chromosome translocations in human peripheral lymphocytes were detected by fluorescence in situ hybridization using 4+# and 7+# combination of composite whole chromosome|specific DNA probes and Giemsa stain.Results The dose|response relationship for chromosome translocation induced by 60 Co γ rays in vitro could be described by the function:Y=0.0030+0 0134D+0 0165D+2.The frequencies of chromosome translocations induced by 0.5 and 2 Gy γ|irradiation did not diminish over time,so it exhibited excellent persistence.Conclusions The results indicate that retrospective dose|reconstruction can be accomplished using chromosome translocation frequency.
{"title":"Fluorescence in situ hybridization detection of chromosome translocations induced by ~(60) Coγ-rays in human lymphocytes dose-response curve and persistence","authors":"Xiaoling Wang, Jin Li","doi":"10.3760/CMA.J.ISSN.0254-5098.2001.02.004","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.0254-5098.2001.02.004","url":null,"abstract":"Objective To study dose|response relationship for chromosome translocation and its persistence measured in human lymphocytes exposed to 60 Co γ rays.Methods The chromosome translocations in human peripheral lymphocytes were detected by fluorescence in situ hybridization using 4+# and 7+# combination of composite whole chromosome|specific DNA probes and Giemsa stain.Results The dose|response relationship for chromosome translocation induced by 60 Co γ rays in vitro could be described by the function:Y=0.0030+0 0134D+0 0165D+2.The frequencies of chromosome translocations induced by 0.5 and 2 Gy γ|irradiation did not diminish over time,so it exhibited excellent persistence.Conclusions The results indicate that retrospective dose|reconstruction can be accomplished using chromosome translocation frequency.","PeriodicalId":374575,"journal":{"name":"Chinese Journal of Radiation Mediation and Protection","volume":"56 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2001-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124765460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2001-03-25DOI: 10.3760/CMA.J.ISSN.0254-5098.2001.02.011
Zhanchun Zhang, Ting Jia, Ying Zhu
Objective To investigate whether or not adaptive response of the 2BS and A549 cells induced by low dose irradiation.Moreover,to analyze the relationship between the adaptive response and cells cycle regulation. Methods 1.The dose survival curves of A549 and 2BS cells were figured by colony formation and typan blue cell staining.2.Flow cytometry was used to analyze the cell cycle after the different doses irradiation. Results 1. The adaptive response induced in 2BS cells by low dose,while it was not induced in A549 cells.2.G 2 phase arrest of 2BS cells after 75mGy X ray plus 4Gy 60 Co γ ray irradiation appeared in 30 minutes,and cell cycle recovered in 24 hours. Conclusion The adaptive response of 2BS cell could be induced by low dose irradiation,and there was close relationship between the adaptive responses and the regulation of cell cycle.
目的观察低剂量辐照诱导2BS和A549细胞是否产生适应性反应。此外，还分析了适应性反应与细胞周期调节的关系。方法 1。采用集落形成法和泰蓝染色法绘制A549和2BS细胞的剂量存活曲线。用流式细胞术分析不同剂量辐照后的细胞周期。结果 1。低剂量对2BS细胞有诱导作用，而对A549细胞无诱导作用。75mGy X射线+ 4Gy 60 Co γ射线照射后，2BS细胞在30分钟内出现g2期阻滞，24小时内细胞周期恢复。结论低剂量辐照可诱导2BS细胞产生适应性反应，且适应性反应与细胞周期调节密切相关。
{"title":"Study on adaptive responses of cells A549 and 2BS induced by low does irradiation","authors":"Zhanchun Zhang, Ting Jia, Ying Zhu","doi":"10.3760/CMA.J.ISSN.0254-5098.2001.02.011","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.0254-5098.2001.02.011","url":null,"abstract":"Objective To investigate whether or not adaptive response of the 2BS and A549 cells induced by low dose irradiation.Moreover,to analyze the relationship between the adaptive response and cells cycle regulation. Methods 1.The dose survival curves of A549 and 2BS cells were figured by colony formation and typan blue cell staining.2.Flow cytometry was used to analyze the cell cycle after the different doses irradiation. Results 1. The adaptive response induced in 2BS cells by low dose,while it was not induced in A549 cells.2.G 2 phase arrest of 2BS cells after 75mGy X ray plus 4Gy 60 Co γ ray irradiation appeared in 30 minutes,and cell cycle recovered in 24 hours. Conclusion The adaptive response of 2BS cell could be induced by low dose irradiation,and there was close relationship between the adaptive responses and the regulation of cell cycle.","PeriodicalId":374575,"journal":{"name":"Chinese Journal of Radiation Mediation and Protection","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2001-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114404642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2001-03-25DOI: 10.3760/CMA.J.ISSN.0254-5098.2001.02.003
B. Su, W. Cai, Cheng-gang Zhang, B. Perbal
Objective To study the relationship between the expression of GAP|43 mRNA and nerve regeneration in rat brain after 60 Co γ|irradiation. Methods Wistar rats were subjected to whole|body irradiation with 8 Gy 60 Co γ|rays.The expression of GAP|43 was detected by in situ hybridization histochemistry using Dig|cRNA probe. Results It was found that the expression of GAP|43 mRNA increased in the cerebral cortex,caudate,putamen,globus pallidus,thalamus and hypothalamus one week after 8 Gy 60 Co γ|irradiation.The peak of GAP|43 mRNA expression was observed in the fourth week and then began to decrease but still remained at a higher than normal level.However,it decreased to a low level after 7 weeks. Conclusion Enhanced expression of GAP|43 mRNA after 60 Co γ|irradiation in rat brain is associated with nerve regeneration and reconstruction of synapse.
目的研究60 Co γ辐照后大鼠脑组织GAP 43 mRNA表达与神经再生的关系。方法采用8 Gy 60 Co γ射线对Wistar大鼠进行全身照射。采用Dig cRNA探针原位杂交组织化学方法检测GAP 43的表达。结果8 Gy 60 Co γ辐照1周后，大鼠大脑皮层、尾状核、壳核、苍白球、丘脑和下丘脑的GAP 43 mRNA表达增加。GAP|43 mRNA表达在第四周达到峰值，随后开始下降，但仍保持在高于正常水平。7周后降至较低水平。结论60 Co γ辐照后大鼠脑组织GAP 43 mRNA表达增强与神经再生和突触重建有关。
{"title":"~(60)Co gamma-irradiation enhances expression of GAP-43 mRNA in rat brain","authors":"B. Su, W. Cai, Cheng-gang Zhang, B. Perbal","doi":"10.3760/CMA.J.ISSN.0254-5098.2001.02.003","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.0254-5098.2001.02.003","url":null,"abstract":"Objective To study the relationship between the expression of GAP|43 mRNA and nerve regeneration in rat brain after 60 Co γ|irradiation. Methods Wistar rats were subjected to whole|body irradiation with 8 Gy 60 Co γ|rays.The expression of GAP|43 was detected by in situ hybridization histochemistry using Dig|cRNA probe. Results It was found that the expression of GAP|43 mRNA increased in the cerebral cortex,caudate,putamen,globus pallidus,thalamus and hypothalamus one week after 8 Gy 60 Co γ|irradiation.The peak of GAP|43 mRNA expression was observed in the fourth week and then began to decrease but still remained at a higher than normal level.However,it decreased to a low level after 7 weeks. Conclusion Enhanced expression of GAP|43 mRNA after 60 Co γ|irradiation in rat brain is associated with nerve regeneration and reconstruction of synapse.","PeriodicalId":374575,"journal":{"name":"Chinese Journal of Radiation Mediation and Protection","volume":"21 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2001-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129955735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2001-03-25DOI: 10.3760/CMA.J.ISSN.0254-5098.2001.02.002
Yun-lai Wang, Liang Zhang
Objective To calculate the mean absorbed doses at celluar and subcellular levels for uniformly and non|uniformly distributed Auger electron emitters. Methods The energy deposited in cell or nucleus by Auger electrons was analytically calculated using the polynomial representation of energy loss.S|values were subsequently computed for several target|source combinations.The absorbed dose and dose distribution were calculated for spherically symmetric radionuclide distributions depending linearly and exponentially on the radial position.The dose profile as a function of the source radial coordinate was also evaluated for typical cell sizes.The contributions of photon radiation to absorbed dose in cells were ignored. Results The mean absorbed dose and dose distribution depend largely on the size of target cells,the radiation spectrum and intracellular localization and distribution of radionuclides.The contribution of intranuclear radionuclides to mean absorbed dose was larger than that of extranuclear ones. Conclusion Auger electrons can produce high local energy deposition in cells because of their very low energies and extremely short ranges.Our dose calculation method is simple.The results are reliable and can be used in many fields.
{"title":"Mean absorbed dose calculation at cellular level for targeted radiotherapy using Auger-electron-emitters","authors":"Yun-lai Wang, Liang Zhang","doi":"10.3760/CMA.J.ISSN.0254-5098.2001.02.002","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.0254-5098.2001.02.002","url":null,"abstract":"Objective To calculate the mean absorbed doses at celluar and subcellular levels for uniformly and non|uniformly distributed Auger electron emitters. Methods The energy deposited in cell or nucleus by Auger electrons was analytically calculated using the polynomial representation of energy loss.S|values were subsequently computed for several target|source combinations.The absorbed dose and dose distribution were calculated for spherically symmetric radionuclide distributions depending linearly and exponentially on the radial position.The dose profile as a function of the source radial coordinate was also evaluated for typical cell sizes.The contributions of photon radiation to absorbed dose in cells were ignored. Results The mean absorbed dose and dose distribution depend largely on the size of target cells,the radiation spectrum and intracellular localization and distribution of radionuclides.The contribution of intranuclear radionuclides to mean absorbed dose was larger than that of extranuclear ones. Conclusion Auger electrons can produce high local energy deposition in cells because of their very low energies and extremely short ranges.Our dose calculation method is simple.The results are reliable and can be used in many fields.","PeriodicalId":374575,"journal":{"name":"Chinese Journal of Radiation Mediation and Protection","volume":"37 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2001-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127851118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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