Antioxidnat Activity and Chemical Profiling of Water Extract of Asimina triloba Leaves

Abstract

In this study, basic characteristics such as radical scavenging activity of the hot water extract of Asimina triloba leaves were confirmed, and qualitative and quantitative analysis of major componunds was conducted. The polyphenol and flavonoid contents of the extract were confirmed to be 94.11 mg/g and 47.28 mg/g. In 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, the extract showed an SC50 of 1.004 mg/mL, which corresponds to a relative activity of 6.77% converted based on the SC50 value of ascorbic acid used as a control. Liquid chromatograph (LC) profile analysis and LC-mass spectrometer (MS)/MS analysis were performed to confirm the major compounds of the extract. As a result of the analysis, a total of five major compounds were identified, including flavonoid such as rutin and isoquercitrin, and chlorogenic acid, cryptochlorogenic acid, and neochlorogenic acid having a caffeoylquinic acid structure. Among these, the compound with the highest content was rutin, which was 59.67 mg/g. Since these compounds are reported to have various physiological activities, it is necessary to basically identify each compound in the process of further research using the leaves of A. triloba.