Biochemical and biomechanical characterization of an autologous protein-based fibrin sealant for regenerative medicine

IF 4.2 3区医学 Q2 ENGINEERING, BIOMEDICAL Journal of Materials Science: Materials in Medicine Pub Date : 2024-03-08 DOI:10.1007/s10856-024-06780-4

Eduardo Anitua, Ander Pino, Roberto Prado, Francisco Muruzabal, Mohammad Hamdan Alkhraisat

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Abstract

Accidental events or surgical procedures usually lead to tissue injury. Fibrin sealants have proven to optimize the healing process but have some drawbacks due to their allogeneic nature. Autologous fibrin sealants present several advantages. The aim of this study is to evaluate the performance of a new autologous fibrin sealant based on Endoret®PRGF® technology (E-sealant). One of the most widely used commercial fibrin sealants (Tisseel®) was included as comparative Control. E-sealant´s hematological and biological properties were characterized. The coagulation kinetics and the microstructure were compared. Their rheological profile and biomechanical behavior were also recorded. Finally, the swelling/shrinkage capacity and the enzymatic degradation of adhesives were determined. E-sealant presented a moderate platelet concentration and physiological levels of fibrinogen and thrombin. It clotted 30 s after activation. The microstructure of E-sealant showed a homogeneous fibrillar scaffold with numerous and scattered platelet aggregates. In contrast, Control presented absence of blood cells and amorphous protein deposits. Although in different order of magnitude, both adhesives had similar rheological profiles and viscoelasticity. Control showed a higher hardness but both adhesives presented a pseudoplastic hydrogel nature with a shear thinning behavior. Regarding their adhesiveness, E-sealant presented a higher tensile strength before cohesive failure but their elastic stretching capacity and maximum elongation was similar. While E-sealant presented a significant shrinkage process, Control showed a slight swelling over time. In addition, E-sealant presented a high enzymatic resorption rate, while Control showed to withstand the biodegradation process in a significant way. E-sealant presents optimal biochemical and biomechanical properties suitable for its use as a fibrin sealant with regenerative purposes.

Graphical Abstract

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用于再生医学的自体蛋白基纤维蛋白密封剂的生物化学和生物力学特征。

意外事件或外科手术通常会导致组织损伤。事实证明，纤维蛋白密封剂可以优化愈合过程，但由于其异体性质，也存在一些缺点。自体纤维蛋白密封剂具有一些优点。本研究旨在评估基于 Endoret®PRGF® 技术的新型自体纤维蛋白密封剂（E-sealant）的性能。其中一种应用最广泛的商用纤维蛋白密封剂（Tisseel®）被列为对比对照组。对 E-sealant 的血液学和生物学特性进行了鉴定。比较了凝固动力学和微观结构。还记录了它们的流变学特征和生物力学行为。最后，还测定了粘合剂的膨胀/收缩能力和酶降解能力。E 型密封剂的血小板浓度适中，纤维蛋白原和凝血酶含量符合生理水平。它在激活后 30 秒就会凝结。E-sealant 的微观结构显示出均匀的纤维支架，其中有大量分散的血小板聚集。相比之下，对照组则没有血细胞和无定形的蛋白质沉淀。虽然数量级不同，但两种粘合剂具有相似的流变曲线和粘弹性。对照组显示出更高的硬度，但两种粘合剂都呈现出具有剪切稀化行为的假塑性水凝胶性质。在粘合性方面，E-密封剂在内聚失效前的拉伸强度更高，但它们的弹性拉伸能力和最大伸长率相似。E 型密封剂会出现明显的收缩过程，而 Control 型密封剂则会随着时间的推移出现轻微的膨胀。此外，E 型密封剂的酶吸收率较高，而对照组则在很大程度上经受住了生物降解过程。E 型密封剂具有最佳的生物化学和生物力学特性，适合用作具有再生功能的纤维蛋白密封剂。

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来源期刊

Journal of Materials Science: Materials in Medicine 工程技术-材料科学：生物材料

CiteScore

8.00

自引率

0.00%

发文量

审稿时长

3.5 months

期刊介绍： The Journal of Materials Science: Materials in Medicine publishes refereed papers providing significant progress in the application of biomaterials and tissue engineering constructs as medical or dental implants, prostheses and devices. Coverage spans a wide range of topics from basic science to clinical applications, around the theme of materials in medicine and dentistry. The central element is the development of synthetic and natural materials used in orthopaedic, maxillofacial, cardiovascular, neurological, ophthalmic and dental applications. Special biomedical topics include biomaterial synthesis and characterisation, biocompatibility studies, nanomedicine, tissue engineering constructs and cell substrates, regenerative medicine, computer modelling and other advanced experimental methodologies.