Biodegradation of polyethylene terephthalate (PET) by Brucella intermedia IITR130 and its proposed metabolic pathway.

IF 3.1 4区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Biodegradation Pub Date : 2024-08-01 Epub Date: 2024-03-09 DOI:10.1007/s10532-024-10070-9

Pallavi Srivastava, Joel Saji, Natesan Manickam

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Abstract

Accumulation of polyethylene terephthalate (PET) polyester in ecosystems across the globe is a major pollution of concern. Microbial degradation recently generated novel insights into the biodegradation of varieties of plastics. In this study, a PET degrading bacterium Brucella intermedia IITR130 was isolated from a contaminated lake ecosystem at Pallikaranai, Chennai, India. Incubation of the bacterium along with the PET sheet (0.1 mm thickness) for 60 days resulted in 26.06% degradation, indicating a half-life of 137.8 days. Considerable changes in the surface morphology of the PET sheet were found as holes, pits, and cracks on incubation with strain IITR130, as revealed by scanning electron microscopy (SEM). After bacterial treatment of PET, the formation of new functional groups, most notably in the area of 3326 cm^-1 suggestive of O-H stretch, leading to carboxylic acid and alcohol as products were suggested by fourier transform infrared (FTIR) analysis. Monomethyl terephthalate (MMT) and terephthalic acid (TPA) were identified by gas chromatography-mass spectrometry (GC-MS) analysis as PET degradation metabolites. Tributyrin clearance assay confirmed the presence of a lipase/esterase enzyme in the strain IITR130. In this study, a degradation pathway for PET by an isolated and identified bacterium Brucella intermedia IITR130 was characterized in detail.

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中间布鲁氏菌 IITR130 对聚对苯二甲酸乙二醇酯（PET）的生物降解及其拟议代谢途径。

聚对苯二甲酸乙二醇酯（PET）聚酯在全球生态系统中的累积是一个令人担忧的重大污染问题。最近，微生物降解对各种塑料的生物降解产生了新的认识。在这项研究中，从印度 Chennai 的 Pallikaranai 受污染的湖泊生态系统中分离出了一种可降解 PET 的中间布鲁氏菌 IITR130。将该细菌与 PET 片材（0.1 毫米厚）一起培养 60 天，降解率为 26.06%，半衰期为 137.8 天。扫描电子显微镜（SEM）显示，在菌株 IITR130 的培养下，PET 片材的表面形态发生了显著变化，出现了孔洞、凹坑和裂缝。傅里叶变换红外（FTIR）分析表明，PET 经细菌处理后，形成了新的官能团，最明显的是在 3326 cm-1 区域，表明存在 O-H 伸展，其产物为羧酸和醇。气相色谱-质谱（GC-MS）分析确定对苯二甲酸单甲酯（MMT）和对苯二甲酸（TPA）为 PET 降解代谢物。三丁炔清除测定证实了 IITR130 菌株中存在脂肪酶/酯酶。本研究详细描述了分离鉴定的中间布鲁氏菌 IITR130 降解 PET 的途径。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biodegradation 工程技术-生物工程与应用微生物

CiteScore

5.60

自引率

0.00%

发文量

审稿时长

6 months

期刊介绍： Biodegradation publishes papers, reviews and mini-reviews on the biotransformation, mineralization, detoxification, recycling, amelioration or treatment of chemicals or waste materials by naturally-occurring microbial strains, microbial associations, or recombinant organisms. Coverage spans a range of topics, including Biochemistry of biodegradative pathways; Genetics of biodegradative organisms and development of recombinant biodegrading organisms; Molecular biology-based studies of biodegradative microbial communities; Enhancement of naturally-occurring biodegradative properties and activities. Also featured are novel applications of biodegradation and biotransformation technology, to soil, water, sewage, heavy metals and radionuclides, organohalogens, high-COD wastes, straight-, branched-chain and aromatic hydrocarbons; Coverage extends to design and scale-up of laboratory processes and bioreactor systems. Also offered are papers on economic and legal aspects of biological treatment of waste.