Pore graded borosilicate bioactive glass scaffolds: in vitro dissolution and cytocompatibility

IF 4.2 3区 医学 Q2 ENGINEERING, BIOMEDICAL Journal of Materials Science: Materials in Medicine Pub Date : 2024-03-20 DOI:10.1007/s10856-024-06791-1
Agata Szczodra, Amel Houaoui, Turkka Salminen, Markus Hannula, Virginia Alessandra Gobbo, Sonya Ghanavati, Susanna Miettinen, Jonathan Massera
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Abstract

3D borosilicate bioactive glass (1393B20 and B12.5MgSr) scaffolds were prepared by robocasting, with and without a dense layer at the top. Pore graded scaffolds are promising as they allow for membrane deposition and could limit the risk of soft tissue infiltration. In vitro dissolution was studied in tris(hydroxymethyl)aminomethane (TRIS) and Simulated Body Fluid (SBF). 1393B20 scaffolds dissolved faster than B12.5MgSr in TRIS whereas they dissolved slower in SBF. The difference in dissolution profiles, as a function of the medium used, is assigned to the different rates of precipitation of hydroxyapatite (HA). While the precipitation of calcium phosphate (CaP) in the form of HA, first sign of bioactivity, was confirmed by ICP, FTIR-ATR and SEM-EDX analysis for both compositions, 1393B20 was found to precipitate HA at a faster rate. The presence of a dense top layer did not significantly impact the dissolution rate and CaP precipitation. In vitro cell culture was performed using human adipose-derived stem cells (hADSCs). Prior to cell plating, a preincubation of 3 days was found optimum to prevent burst ion release. In direct contact, cells proliferate and spread on the scaffolds while maintaining characteristic spindle morphology. Cell plated on 1393B20 scaffolds showed increased viability when compared to cell plated on B12.5MgSr. The lower cell viability, when testing B12.5MgSr, was assigned to the depletion of Ca2+ ions from culture medium and higher pH. Static cell culture leads to believe that the scaffold produced from the 1393B20 glass composition are promising in bone regeneration applications.

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孔分级硼硅酸盐生物活性玻璃支架:体外溶解和细胞相容性。
通过机械铸造法制备了三维硼硅酸盐生物活性玻璃(1393B20 和 B12.5MgSr)支架,支架顶部有致密层和无致密层。孔隙分级支架很有前景,因为它们允许膜沉积,并能限制软组织浸润的风险。在三(羟甲基)氨基甲烷(TRIS)和模拟体液(SBF)中对体外溶解进行了研究。1393B20 支架在 TRIS 中的溶解速度比 B12.5MgSr 快,而在 SBF 中的溶解速度较慢。溶解曲线的差异与所用介质有关,这是因为羟基磷灰石(HA)的沉淀速度不同。通过对两种成分进行 ICP、FTIR-ATR 和 SEM-EDX 分析,以 HA 形式沉淀的磷酸钙(CaP)是生物活性的第一个标志,但 1393B20 的 HA 沉淀速度更快。致密顶层的存在对溶解速率和 CaP 沉淀没有明显影响。体外细胞培养使用的是人脂肪干细胞(hADSCs)。在细胞培养前,发现预孵育 3 天是防止猝灭离子释放的最佳时间。在直接接触中,细胞在支架上增殖和扩散,同时保持特有的纺锤体形态。与在 B12.5MgSr 上培养的细胞相比,在 1393B20 支架上培养的细胞存活率更高,而在 B12.5MgSr 上培养的细胞存活率较低,原因是培养基中的 Ca2+ 离子耗尽和 pH 值升高。静态细胞培养使我们相信,由 1393B20 玻璃成分制成的支架在骨再生应用中大有可为。
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来源期刊
Journal of Materials Science: Materials in Medicine
Journal of Materials Science: Materials in Medicine 工程技术-材料科学:生物材料
CiteScore
8.00
自引率
0.00%
发文量
73
审稿时长
3.5 months
期刊介绍: The Journal of Materials Science: Materials in Medicine publishes refereed papers providing significant progress in the application of biomaterials and tissue engineering constructs as medical or dental implants, prostheses and devices. Coverage spans a wide range of topics from basic science to clinical applications, around the theme of materials in medicine and dentistry. The central element is the development of synthetic and natural materials used in orthopaedic, maxillofacial, cardiovascular, neurological, ophthalmic and dental applications. Special biomedical topics include biomaterial synthesis and characterisation, biocompatibility studies, nanomedicine, tissue engineering constructs and cell substrates, regenerative medicine, computer modelling and other advanced experimental methodologies.
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