Biocompatibility of nano/micro-sized pyrophyllite particles by pulmo, liver, kidney and gastric mucosis cells

IF 4.2 3区医学 Q2 ENGINEERING, BIOMEDICAL Journal of Materials Science: Materials in Medicine Pub Date : 2024-06-17 DOI:10.1007/s10856-024-06793-z

Smiljana Paraš, Jovana Paspalj, Karima Baghdad, Ognjenka Janković, Ranko Škrbić, Radoslav Gajanin, Pascale Massiani, Franck Launay, Suzana Gotovac Atlagić

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Abstract

Pyrophyllite is the least studied natural clay in terms of its potential in biomedical applications, although there are many deposits of this aluminosilicate around the world. Genotoxicity study was performed in vitro for this mineral. Subsequently, Wister rats were exposed to the pyrophyllite micronized to below 100 µm. After the exposure period, histology of the lung, liver, kidney and gastric tissues were performed, followed by the stereological and hematological analysis. The physicochemical analyses revealed typical XRD characteristics of pyrophyllite clay with particle-size distribution ranging 50 nm–100 μm with stable mineral composition and unique buffering property to pH around 8. The results showed that there were no cytotoxic effects on to THP-1 cells, or genotoxicity of pyrophyllite measured by the Comet assay. In vivo studies are accompanied by the thorough physicochemical characterization of the micronized pyrophyllite. Histology of the lung tissue proved presence of an inflammatory reaction. On the other hand, gastric tissue has shown the selective accumulation of nanoparticles in enterocytes of the stomach only, as supported by ultrastructural analysis. Liver and kidney tissues have shown tolerability for pyrophyllite particles. The results give directions for further comprehensive studies of potential biomedical applications of the pyrophyllite.

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肺、肝、肾和胃黏膜细胞对纳米/微米大小的辉绿岩颗粒的生物相容性。

尽管全世界有许多这种铝硅酸盐的矿藏，但就其在生物医学应用方面的潜力而言，辉绿岩是研究最少的天然粘土。对这种矿物进行了体外遗传毒性研究。随后，Wister 大鼠接触了微粉化至 100 微米以下的辉绿岩。接触期结束后，对大鼠的肺、肝、肾和胃组织进行了组织学检查，然后进行了立体学和血液学分析。理化分析表明，辉绿岩粘土具有典型的 XRD 特性，粒度分布范围为 50 nm-100 μm，矿物成分稳定，具有独特的缓冲特性，pH 值在 8 左右。研究结果表明，辉绿岩对 THP-1 细胞没有细胞毒性作用，也没有彗星试验测定的遗传毒性。在进行体内研究的同时，还对微粉化的叶蜡石进行了全面的物理化学鉴定。肺组织的组织学检查证明存在炎症反应。另一方面，胃组织的超微结构分析表明，纳米颗粒只选择性地聚集在胃的肠细胞中。肝脏和肾脏组织显示出对辉绿岩颗粒的耐受性。这些结果为进一步全面研究辉绿岩的潜在生物医学应用提供了方向。

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来源期刊

Journal of Materials Science: Materials in Medicine 工程技术-材料科学：生物材料

CiteScore

8.00

自引率

0.00%

发文量

审稿时长

3.5 months

期刊介绍： The Journal of Materials Science: Materials in Medicine publishes refereed papers providing significant progress in the application of biomaterials and tissue engineering constructs as medical or dental implants, prostheses and devices. Coverage spans a wide range of topics from basic science to clinical applications, around the theme of materials in medicine and dentistry. The central element is the development of synthetic and natural materials used in orthopaedic, maxillofacial, cardiovascular, neurological, ophthalmic and dental applications. Special biomedical topics include biomaterial synthesis and characterisation, biocompatibility studies, nanomedicine, tissue engineering constructs and cell substrates, regenerative medicine, computer modelling and other advanced experimental methodologies.