Rapid and high-throughput determination of sorghum (Sorghum bicolor) biomass composition using near infrared spectroscopy and chemometrics

IF 5.8 2区生物学 Q1 AGRICULTURAL ENGINEERING Biomass & Bioenergy Pub Date : 2024-06-19 DOI:10.1016/j.biombioe.2024.107276

Md Wadud Ahmed , Carlos A. Esquerre , Kristen Eilts , Dylan P. Allen , Scott M. McCoy , Sebastian Varela , Vijay Singh , Andrew D.B. Leakey , Mohammed Kamruzzaman

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Abstract

Compositional characterization of biomass is vital for the biofuel industry. Traditional wet chemistry-based methods for analyzing biomass composition are laborious, time-consuming, and require extensive use of chemical reagents as well as highly skilled personnel. In this study, near-infrared (NIR) spectroscopy was used to quickly assess the composition of above-ground vegetative biomass from 113 diverse, photoperiod-sensitive, biomass-type sorghum (Sorghum bicolor) accessions cultivated under field conditions in Central Illinois. Biomass samples were analyzed using NIR spectra collected in the spectral range of 867–2536 nm, with their chemical compositions determined following the National Renewable Energy Laboratory (NREL) protocol. Advanced spectral pre-treatment and band selection techniques were utilized to develop calibration models using partial least squares regression (PLSR). The models' effectiveness was assessed through cross-validation and independent data tests. The predictions for moisture, ash, extractives, glucan, xylan, acid-soluble lignin (ASL), acid-insoluble lignin (AIL), and total lignin were accurate and reliable, demonstrating the capability of NIR spectroscopy to provide rapid and precise characterization of sorghum biomass. The results demonstrated that NIR spectroscopy is an efficient tool for rapidly characterizing sorghum biomass, making it a sustainable option for screening desirable feedstock for biofuel or bioproduct production.

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利用近红外光谱和化学计量学快速、高通量测定高粱（Sorghum bicolor）的生物质成分

生物质的成分表征对生物燃料工业至关重要。传统的基于湿化学的生物质成分分析方法费时费力，需要大量化学试剂和高技能人才。本研究利用近红外光谱快速评估了伊利诺伊州中部田间条件下栽培的 113 种不同的、对光周期敏感的生物质型高粱（Sorghum bicolor）地上植被生物量的组成。生物质样本使用在 867-2536 纳米光谱范围内采集的近红外光谱进行分析，并按照美国国家可再生能源实验室（NREL）的规程测定其化学成分。利用先进的光谱预处理和波段选择技术，使用偏最小二乘法回归 (PLSR) 建立了校准模型。通过交叉验证和独立数据测试评估了模型的有效性。对水分、灰分、萃取物、葡聚糖、木聚糖、酸溶性木质素（ASL）、酸不溶性木质素（AIL）和总木质素的预测准确可靠，证明了近红外光谱技术能够快速、精确地表征高粱生物质。研究结果表明，近红外光谱仪是快速表征高粱生物质的有效工具，使其成为筛选生物燃料或生物产品生产所需原料的可持续选择。

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来源期刊

Biomass & Bioenergy 工程技术-能源与燃料

CiteScore

11.50

自引率

3.30%

发文量

258

审稿时长

60 days

期刊介绍： Biomass & Bioenergy is an international journal publishing original research papers and short communications, review articles and case studies on biological resources, chemical and biological processes, and biomass products for new renewable sources of energy and materials. The scope of the journal extends to the environmental, management and economic aspects of biomass and bioenergy. Key areas covered by the journal: • Biomass: sources, energy crop production processes, genetic improvements, composition. Please note that research on these biomass subjects must be linked directly to bioenergy generation. • Biological Residues: residues/rests from agricultural production, forestry and plantations (palm, sugar etc), processing industries, and municipal sources (MSW). Papers on the use of biomass residues through innovative processes/technological novelty and/or consideration of feedstock/system sustainability (or unsustainability) are welcomed. However waste treatment processes and pollution control or mitigation which are only tangentially related to bioenergy are not in the scope of the journal, as they are more suited to publications in the environmental arena. Papers that describe conventional waste streams (ie well described in existing literature) that do not empirically address ''new'' added value from the process are not suitable for submission to the journal. • Bioenergy Processes: fermentations, thermochemical conversions, liquid and gaseous fuels, and petrochemical substitutes • Bioenergy Utilization: direct combustion, gasification, electricity production, chemical processes, and by-product remediation • Biomass and the Environment: carbon cycle, the net energy efficiency of bioenergy systems, assessment of sustainability, and biodiversity issues.