Enzymatic hydrolysis of chitinous wastes pretreated by deep eutectic solvents into N-acetyl glucosamine

IF 6.3 2区化学 Q1 POLYMER SCIENCE Polymer Degradation and Stability Pub Date : 2024-06-27 DOI:10.1016/j.polymdegradstab.2024.110907

Xi Zhou , Quanzhen Liu , Xueman Chen , Ning Zhou , Guoguang Wei , Feifei Chen , Alei Zhang , Kequan Chen

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Abstract

In this study, we present an efficient and green extraction-pretreatment integrated approach for enhancing enzymatic conversion of chitinous wastes into N-acetyl-d-glucosamine (GlcNAc). Firstly, the enzyme cocktail containing a chitinase CmChi1 and a N-acetyl glucosaminase CmNAGase were constructed for hydrolyzing chitin into sole GlcNAc. Secondly, deep eutectic solvent (DES), consisting of choline chloride and glycollic acid was used to treat chitinous wastes. Under optimal conditions, chitin yield reach to 72 % with a purity of 98 %. Fourier-transform infrared spectroscopy, thermogravimetric analysis, and X-ray diffraction analysis revealed that the crystallinity and thermal stability of the obtained chitin decreased upon DES treatment without alteration of the chemical structure or deacetylation. Finally, the concentration of GlcNAc was increased 2–6 folds by enzymatic hydrolysis of DES-treated chitinous wastes (including shrimp shell, crab shell, ganoderma spores wall, and mycelium). The process provides a promising strategy for degrading chitinous wastes to produce high valued GlcNAc.

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甲壳质废料经深共晶溶剂预处理后酶水解为 N-乙酰葡糖胺

在本研究中，我们提出了一种高效、绿色的提取-预处理一体化方法，用于提高甲壳质废料向 N-乙酰-d-葡糖胺（GlcNAc）的酶转化率。首先，我们构建了包含甲壳素酶 CmChi1 和 N-乙酰葡糖胺酶 CmNAGase 的鸡尾酒酶，用于将甲壳素水解为唯一的 GlcNAc。其次，利用由氯化胆碱和甘醇酸组成的深共晶溶剂（DES）处理甲壳质废料。在最佳条件下，甲壳素产量达到 72%，纯度为 98%。傅立叶变换红外光谱分析、热重分析和 X 射线衍射分析表明，DES 处理后甲壳素的结晶度和热稳定性都有所下降，但化学结构和脱乙酰度没有发生变化。最后，通过酶水解 DES 处理过的几丁质废弃物（包括虾壳、蟹壳、灵芝孢子壁和菌丝体），GlcNAc 的浓度增加了 2-6 倍。该工艺为降解几丁质废料以生产高价值的 GlcNAc 提供了一种前景广阔的策略。

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来源期刊

Polymer Degradation and Stability 化学-高分子科学

CiteScore

10.10

自引率

10.20%

发文量

325

审稿时长

23 days

期刊介绍： Polymer Degradation and Stability deals with the degradation reactions and their control which are a major preoccupation of practitioners of the many and diverse aspects of modern polymer technology. Deteriorative reactions occur during processing, when polymers are subjected to heat, oxygen and mechanical stress, and during the useful life of the materials when oxygen and sunlight are the most important degradative agencies. In more specialised applications, degradation may be induced by high energy radiation, ozone, atmospheric pollutants, mechanical stress, biological action, hydrolysis and many other influences. The mechanisms of these reactions and stabilisation processes must be understood if the technology and application of polymers are to continue to advance. The reporting of investigations of this kind is therefore a major function of this journal. However there are also new developments in polymer technology in which degradation processes find positive applications. For example, photodegradable plastics are now available, the recycling of polymeric products will become increasingly important, degradation and combustion studies are involved in the definition of the fire hazards which are associated with polymeric materials and the microelectronics industry is vitally dependent upon polymer degradation in the manufacture of its circuitry. Polymer properties may also be improved by processes like curing and grafting, the chemistry of which can be closely related to that which causes physical deterioration in other circumstances.