Electrophysiological Insights into Antibiotic Translocation and Resistance: The Impact of Outer Membrane Proteins.

IF 3.3 4区 工程技术 Q2 CHEMISTRY, PHYSICAL Membranes Pub Date : 2024-07-20 DOI:10.3390/membranes14070161
Ishan Ghai
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Abstract

The alarming rise of antibiotic resistance in Gram-negative bacteria has emerged as a major global health challenge. A key factor contributing to this crisis is the low permeability of the bacterial outer membrane, which acts as a barrier that prevents antibiotics from entering the cell. Protein channels embedded in this outer membrane selectively regulate the influx of hydrophilic compounds, including antibiotics. To combat antibiotic resistance, understanding the molecular mechanisms governing antibiotic permeability through bacterial membrane channels is crucial. This knowledge is key towards elucidating their roles in studing antibiotic resistance. By compiling and analysing the flux data from multiple electrophysiological reversal potential experimental studies, which involves measuring zero-current potentials and the corresponding single-channel conductance, we can calculate the flux of charged antibiotics/compounds across different Gram-negative bacterial outer membrane channels. Through this comprehensive synthesis, this review aims to advance our understanding and stimulate discussions about the physicochemical factors influencing the flux of antibiotics through bacterial membrane protein channels, ultimately enhancing our knowledge in this area.

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抗生素转运和耐药性的电生理学启示:外膜蛋白的影响。
革兰氏阴性细菌中抗生素耐药性的惊人增长已成为全球健康的一大挑战。导致这一危机的一个关键因素是细菌外膜的低渗透性,它是阻止抗生素进入细胞的屏障。嵌入外膜的蛋白通道可选择性地调节亲水性化合物(包括抗生素)的流入。要对抗抗生素耐药性,了解通过细菌膜通道调节抗生素渗透性的分子机制至关重要。这些知识对于阐明它们在研究抗生素耐药性方面的作用至关重要。通过汇编和分析多项电生理反转电位实验研究的通量数据(包括测量零电流电位和相应的单通道电导),我们可以计算出带电抗生素/化合物通过不同革兰氏阴性细菌外膜通道的通量。这篇综述旨在通过全面的综述,加深我们对影响抗生素通过细菌膜蛋白通道的物理化学因素的理解并激发讨论,最终增进我们在这一领域的知识。
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来源期刊
Membranes
Membranes Chemical Engineering-Filtration and Separation
CiteScore
6.10
自引率
16.70%
发文量
1071
审稿时长
11 weeks
期刊介绍: Membranes (ISSN 2077-0375) is an international, peer-reviewed open access journal of separation science and technology. It publishes reviews, research articles, communications and technical notes. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. Full experimental and/or methodical details must be provided.
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