Weiwei Yue, Yuwei Cao, Shuqi Xie, Kang Ning Cheng, Yue Ding, Cong Liu, Yan Jing Ding, Xiaofeng Zhu, Huanqing Liu, Muhammad Shafi
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引用次数: 0
Abstract
Purpose
This study aims to improve detection efficiency of fluorescence biosensor or a graphene field-effect transistor biosensor. Graphene field-effect transistor biosensing and fluorescent biosensing were integrated and combined with magnetic nanoparticles to construct a multi-sensor integrated microfluidic biochip for detecting single-stranded DNA. Multi-sensor integrated biochip demonstrated higher detection reliability for a single target and could simultaneously detect different targets.
Design/methodology/approach
In this study, the authors integrated graphene field-effect transistor biosensing and fluorescent biosensing, combined with magnetic nanoparticles, to fabricate a multi-sensor integrated microfluidic biochip for the detection of single-stranded deoxyribonucleic acid (DNA). Graphene films synthesized through chemical vapor deposition were transferred onto a glass substrate featuring two indium tin oxide electrodes, thus establishing conductive channels for the graphene field-effect transistor. Using π-π stacking, 1-pyrenebutanoic acid succinimidyl ester was immobilized onto the graphene film to serve as a medium for anchoring the probe aptamer. The fluorophore-labeled target DNA subsequently underwent hybridization with the probe aptamer, thereby forming a fluorescence detection channel.
Findings
This paper presents a novel approach using three channels of light, electricity and magnetism for the detection of single-stranded DNA, accompanied by the design of a microfluidic detection platform integrating biosensor chips. Remarkably, the detection limit achieved is 10 pm, with an impressively low relative standard deviation of 1.007%.
Originality/value
By detecting target DNA, the photo-electro-magnetic multi-sensor graphene field-effect transistor biosensor not only enhances the reliability and efficiency of detection but also exhibits additional advantages such as compact size, affordability, portability and straightforward automation. Real-time display of detection outcomes on the host facilitates a deeper comprehension of biochemical reaction dynamics. Moreover, besides detecting the same target, the sensor can also identify diverse targets, primarily leveraging the penetrative and noninvasive nature of light.
目的 本研究旨在提高荧光生物传感器或石墨烯场效应晶体管生物传感器的检测效率。将石墨烯场效应晶体管生物传感和荧光生物传感与磁性纳米粒子相结合,构建了用于检测单链 DNA 的多传感器集成微流控生物芯片。设计/方法/方法在这项研究中,作者将石墨烯场效应晶体管生物传感和荧光生物传感与磁性纳米粒子相结合,制造出一种用于检测单链脱氧核糖核酸(DNA)的多传感器集成微流控生物芯片。通过化学气相沉积合成的石墨烯薄膜被转移到具有两个氧化铟锡电极的玻璃基板上,从而为石墨烯场效应晶体管建立了导电通道。通过π-π堆叠,1-芘丁酸琥珀酰亚胺酯被固定在石墨烯薄膜上,作为锚定探针合体的介质。本文提出了一种利用光、电、磁三通道检测单链 DNA 的新方法,并设计了一种集成生物传感器芯片的微流控检测平台。原创性/价值 通过检测目标 DNA,光电磁多传感器石墨烯场效应晶体管生物传感器不仅提高了检测的可靠性和效率,还具有体积小、价格低、便于携带和直接自动化等优点。在主机上实时显示检测结果有助于更深入地了解生化反应动态。此外,除了检测同一目标,该传感器还能识别不同目标,这主要是利用了光的穿透性和非侵入性。
期刊介绍:
Sensor Review publishes peer reviewed state-of-the-art articles and specially commissioned technology reviews. Each issue of this multidisciplinary journal includes high quality original content covering all aspects of sensors and their applications, and reflecting the most interesting and strategically important research and development activities from around the world. Because of this, readers can stay at the very forefront of high technology sensor developments.
Emphasis is placed on detailed independent regular and review articles identifying the full range of sensors currently available for specific applications, as well as highlighting those areas of technology showing great potential for the future. The journal encourages authors to consider the practical and social implications of their articles.
All articles undergo a rigorous double-blind peer review process which involves an initial assessment of suitability of an article for the journal followed by sending it to, at least two reviewers in the field if deemed suitable.
Sensor Review’s coverage includes, but is not restricted to:
Mechanical sensors – position, displacement, proximity, velocity, acceleration, vibration, force, torque, pressure, and flow sensors
Electric and magnetic sensors – resistance, inductive, capacitive, piezoelectric, eddy-current, electromagnetic, photoelectric, and thermoelectric sensors
Temperature sensors, infrared sensors, humidity sensors
Optical, electro-optical and fibre-optic sensors and systems, photonic sensors
Biosensors, wearable and implantable sensors and systems, immunosensors
Gas and chemical sensors and systems, polymer sensors
Acoustic and ultrasonic sensors
Haptic sensors and devices
Smart and intelligent sensors and systems
Nanosensors, NEMS, MEMS, and BioMEMS
Quantum sensors
Sensor systems: sensor data fusion, signals, processing and interfacing, signal conditioning.