Design and application of microfluidics in aptamer SELEX and Aptasensors

IF 12.1 1区工程技术 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Biotechnology advances Pub Date : 2024-10-05 DOI:10.1016/j.biotechadv.2024.108461

Shikun Zhang , Yingming Zhang , Zhiyuan Ning , Mengxia Duan , Xianfeng Lin , Nuo Duan , Zhouping Wang , Shijia Wu

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Abstract

Aptamers are excellent recognition molecules obtained from systematic evolution of ligands by exponential enrichment (SELEX) that have been extensively researched for constructing aptasensors. However, in the process from SELEX to the construction of aptasensors, there are many disadvantages, such as tedious and repetitive operations, interference from external factors, and low efficiency, which seriously limits their application scope and development. Introducing the microfluidic technology can realize the integration and intelligence of SELEX and aptasensing, improve the efficiency of SELEX, and enhance the detection performance and convenience of aptasensing. Hence, in this review, the characteristics of various chips based on different driving forces are described firstly. And then summarizing the design of microfluidic devices based on different SELEX methods and showing the strategies of microfluidic aptasensors based on different detection modes. Finally, discussing the difficulties and challenges encountered when microfluidic is integrated with the SELEX and the aptasensors.

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微流控技术在适配体 SELEX 和适配体传感器中的设计与应用。

适配体是通过指数富集配体系统进化（SELEX）获得的优良识别分子，在构建适配传感器方面得到了广泛的研究。然而，从 SELEX 筛选到构建相应传感器的过程中，存在许多缺点，如操作繁琐重复、受外界因素干扰、效率低等，严重限制了其应用范围和发展。引入微流控技术可以实现 SELEX 和适配体检测的自动化和智能化，提高 SELEX 的效率，增强适配体检测的检测性能和便利性。因此，本综述首先介绍了基于不同驱动力的各种芯片的特点。然后总结了基于不同 SELEX 方法的微流控装置的设计，并展示了基于不同检测模式的微流控适配体的策略。最后，讨论了微流控与适配体 SELEX 和适配体传感器集成时遇到的困难和挑战。

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来源期刊

Biotechnology advances 工程技术-生物工程与应用微生物

CiteScore

25.50

自引率

2.50%

发文量

167

审稿时长

37 days

期刊介绍： Biotechnology Advances is a comprehensive review journal that covers all aspects of the multidisciplinary field of biotechnology. The journal focuses on biotechnology principles and their applications in various industries, agriculture, medicine, environmental concerns, and regulatory issues. It publishes authoritative articles that highlight current developments and future trends in the field of biotechnology. The journal invites submissions of manuscripts that are relevant and appropriate. It targets a wide audience, including scientists, engineers, students, instructors, researchers, practitioners, managers, governments, and other stakeholders in the field. Additionally, special issues are published based on selected presentations from recent relevant conferences in collaboration with the organizations hosting those conferences.