Myosin-binding protein 13 mediates primary seed dormancy via abscisic acid biosynthesis and signaling in Arabidopsis.

IF 6.2 1区 生物学 Q1 PLANT SCIENCES The Plant Journal Pub Date : 2024-10-30 DOI:10.1111/tpj.17112
Cui-Hong Hao, Chen Pang, Li-Na Yang, Feng Xiong, Sha Li
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Abstract

Dormancy is an essential characteristic that enables seeds to survive in unfavorable conditions while germinating when conditions are favorable. Myosin-binding proteins (MyoBs) assist in the movement of organelles along actin microfilaments by attaching to both organelles and myosins. In contrast to studies on yeast and metazoans, research on plant MyoBs is still in its early stages and primarily focuses on tip-growing cells. In this study, we found that Arabidopsis MyoB13 is highly expressed in dry mature seeds. The myob13 mutant, created using CRISPR/Cas9, exhibits a preharvest sprouting phenotype, which can be mitigated by after-ripening treatment, indicating that MyoB13 plays a positive role in primary seed dormancy. Furthermore, we show that MyoB13 negatively regulates ABA biosynthesis and signaling pathways. Notably, the expression of MyoB13 orthologs from maize and soybean can completely restore the phenotype of the Arabidopsis myob13 mutant, suggesting that the function of MyoB13 in ABA-induced seed dormancy is evolutionarily conserved. Therefore, the functional characterization of MyoB13 offers an additional genetic resource to help prevent vivipary in crop species.

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肌球蛋白结合蛋白 13 在拟南芥中通过脱落酸的生物合成和信号传导介导初级种子休眠。
休眠是种子能够在不利条件下存活并在有利条件下萌发的一个基本特征。肌球蛋白结合蛋白(MyoBs)通过附着在细胞器和肌球蛋白上,帮助细胞器沿着肌动蛋白微丝运动。与对酵母和元气动物的研究不同,对植物 MyoBs 的研究仍处于早期阶段,主要集中在顶端生长细胞。在这项研究中,我们发现拟南芥 MyoB13 在干燥成熟的种子中高度表达。利用CRISPR/Cas9技术创建的myob13突变体表现出收获前萌发的表型,这种表型可以通过后熟处理得到缓解,这表明MyoB13在初级种子休眠中发挥着积极作用。此外,我们还发现 MyoB13 负向调节 ABA 的生物合成和信号通路。值得注意的是,表达玉米和大豆的 MyoB13 同源物可以完全恢复拟南芥 myob13 突变体的表型,这表明 MyoB13 在 ABA 诱导的种子休眠中的功能在进化上是保守的。因此,MyoB13的功能特征描述为帮助作物物种防止胎生提供了额外的遗传资源。
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来源期刊
The Plant Journal
The Plant Journal 生物-植物科学
CiteScore
13.10
自引率
4.20%
发文量
415
审稿时长
2.3 months
期刊介绍: Publishing the best original research papers in all key areas of modern plant biology from the world"s leading laboratories, The Plant Journal provides a dynamic forum for this ever growing international research community. Plant science research is now at the forefront of research in the biological sciences, with breakthroughs in our understanding of fundamental processes in plants matching those in other organisms. The impact of molecular genetics and the availability of model and crop species can be seen in all aspects of plant biology. For publication in The Plant Journal the research must provide a highly significant new contribution to our understanding of plants and be of general interest to the plant science community.
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