Combination of PAMP-induced peptide signaling and its regulator SpWRKY65 boosts tomato resistance to Phytophthora infestans

IF 6.2 1区 生物学 Q1 PLANT SCIENCES The Plant Journal Pub Date : 2025-03-16 DOI:10.1111/tpj.70098
Ruirui Yang, Chenglin Su, Zhiyuan Xue, Hongbo Wei, Zhengjie Wang, Jiaxuan Zhu, Jun Meng, Yushi Luan
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Abstract

Late blight, caused by Phytophthora infestans (P. infestans), seriously compromises tomato growth and yield. PAMP-induced peptides (PIPs) are secreted peptides that act as endogenous elicitors, triggering plant immune responses. Our previous research indicated that the exogenous application of PIP1 from Solanum pimpinelifolium L3708, named SpPIP1, enhances tomato resistance to P. infestans. However, little is known about the roles of additional family members in tomato resistance to P. infestans. In addition, there remains a significant gap in understanding the receptors of SpPIPs and the transcription factors (TFs) that regulate SpPIPs signaling in tomato defense, and the combination of SpPIPs signaling and TFs in defending against pathogens is rarely studied. This study demonstrates that the exogenous application of SpPIP-LIKE1 (SpPIPL1) also strengthens tomato resistance by affecting the phenylpropanoid biosynthesis pathway. Both SpPIP1 and SpPIPL1 trigger plant defense responses in a manner dependent on RLK7L. Tomato plants overexpressing the precursors of SpPIP1 and SpPIPL1 (SpprePIP1 and SpprePIPL1) exhibited enhanced expression of pathogenesis-related genes, elevated H2O2 and ABA levels, and increased lignin accumulation. Notably, SpWRKY65 was identified as a transcriptional activator of SpprePIP1 and SpprePIPL1. Disease resistance assays and gene expression analyses revealed that overexpression of SpWRKY65 (OEWRKY65) confers tomato resistance to P. infestans, while wrky65 knockout led to the opposite effect. Intriguingly, transgenic tomato studies showed that either spraying OEWRKY65 with SpPIPs or co-overexpressing SpprePIP1 and SpWRKY65 further augmented tomato resistance, underscoring the potential of gene stacking in enhancing disease resistance. In summary, this study offers new perspectives on controlling late blight and developing tomato varieties with improved resistance. The results emphasize the potential of exogenous SpPIPs application as an eco-friendly strategy for crop protection, laying a theoretical foundation for advancing crop breeding.

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由疫霉(Phytophthora infestans)引起的晚疫病严重影响番茄的生长和产量。PAMP 诱导肽(PIPs)是一种分泌肽,可作为内源诱导剂,引发植物免疫反应。我们之前的研究表明,外源应用来自 Solanum pimpinelifolium L3708 的 PIP1(名为 SpPIP1)可增强番茄对 P. infestans 的抗性。然而,人们对其他家族成员在番茄抗虫害中的作用知之甚少。此外,人们对 SpPIPs 的受体和调控 SpPIPs 信号转导的转录因子(TFs)在番茄防御中的作用的了解仍有很大差距,而 SpPIPs 信号转导和 TFs 在防御病原体中的结合作用也很少有人研究。本研究证明,外源施用 SpPIP-LIKE1 (SpPIPL1)还能通过影响苯丙类生物合成途径增强番茄的抗性。SpPIP1 和 SpPIPL1 都以依赖 RLK7L 的方式触发植物防御反应。过表达 SpPIP1 和 SpPIPL1 的前体(SpprePIP1 和 SpprePIPL1)的番茄植株表现出病原相关基因表达增强、H2O2 和 ABA 水平升高以及木质素积累增加。值得注意的是,SpWRKY65 被鉴定为 SpprePIP1 和 SpprePIPL1 的转录激活因子。抗病性测定和基因表达分析表明,SpWRKY65(OEWRKY65)的过表达可使番茄对侵染性蚜虫产生抗性,而 wrky65 的敲除则会产生相反的效果。耐人寻味的是,转基因番茄研究表明,将 OEWRKY65 与 SpPIPs 一起喷洒或共同表达 SpprePIP1 和 SpWRKY65 都能进一步增强番茄的抗病性,这凸显了基因堆叠在增强抗病性方面的潜力。总之,本研究为控制晚疫病和开发抗性更强的番茄品种提供了新的视角。研究结果强调了外源 SpPIPs 作为一种生态友好型作物保护策略的应用潜力,为推进作物育种奠定了理论基础。
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来源期刊
The Plant Journal
The Plant Journal 生物-植物科学
CiteScore
13.10
自引率
4.20%
发文量
415
审稿时长
2.3 months
期刊介绍: Publishing the best original research papers in all key areas of modern plant biology from the world"s leading laboratories, The Plant Journal provides a dynamic forum for this ever growing international research community. Plant science research is now at the forefront of research in the biological sciences, with breakthroughs in our understanding of fundamental processes in plants matching those in other organisms. The impact of molecular genetics and the availability of model and crop species can be seen in all aspects of plant biology. For publication in The Plant Journal the research must provide a highly significant new contribution to our understanding of plants and be of general interest to the plant science community.
期刊最新文献
Genetic engineering in diatoms: advances and prospects Navigating the challenges of engineering composite specialized metabolite pathways in plants Combination of PAMP-induced peptide signaling and its regulator SpWRKY65 boosts tomato resistance to Phytophthora infestans Decoding genetic diversity through genome engineering in bryophytes Auxin-responsive OsMADS60 negatively mediates rice tillering and grain yield by modulating OsPIN5b expression
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