{"title":"Improving Macromolecule Crowding Configurations in Nanopores for Protein Sensing","authors":"Fei Zheng, HongLuan Li, Jun Yang, Haiyan Wang, Guangle Qin, Dapeng Chen, Jingjie Sha","doi":"10.1039/d4cc05344c","DOIUrl":null,"url":null,"abstract":"We show that PEG-induced macromolecular crowding enhances protein detection in nanopores by increasing capture rate and translocation frequency. Experimental data indicate that a PEG concentration gradient boosts capture efficiency, while our theoretical model attributes this enhancement to osmotic flow, offering insights for improving nanopore-based biosensing.","PeriodicalId":67,"journal":{"name":"Chemical Communications","volume":null,"pages":null},"PeriodicalIF":4.3000,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Chemical Communications","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1039/d4cc05344c","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}
引用次数: 0
Abstract
We show that PEG-induced macromolecular crowding enhances protein detection in nanopores by increasing capture rate and translocation frequency. Experimental data indicate that a PEG concentration gradient boosts capture efficiency, while our theoretical model attributes this enhancement to osmotic flow, offering insights for improving nanopore-based biosensing.
期刊介绍：
ChemComm (Chemical Communications) is renowned as the fastest publisher of articles providing information on new avenues of research, drawn from all the world''s major areas of chemical research.
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