A novel bioactive and functional exopolysaccharide from the cyanobacterial strain Arthrospira maxima cultivated under salinity stress.

IF 3.5 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Bioprocess and Biosystems Engineering Pub Date : 2024-12-17 DOI:10.1007/s00449-024-03120-2

Amel Harbaoui, Nadia Khelifi, Neyssene Aissaoui, Murielle Muzard, Agathe Martinez, Issam Smaali

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Abstract

Cyanobacterial exopolysaccharides (EPS) remain released by cyanobacteria in the surrounding environment with the main purpose of protection against harmful environmental conditions. Recently, they have received significant attention due to their unique structural characteristics, functional properties, and potential applications across various fields. The current study describes the evaluation of EPS production under salinity stress from Arthrospira maxima. The application of high salinity up to 40 g/L enhanced EPS production, which was collected and purified by alcohol precipitation followed by membrane dialysis and lyophilization. A yield of 60 mg/L was obtained. The Size exclusion chromatography gave for the purified EPS an apparent molecular weight of 2.1 × 10⁵ Da. Monosaccharide composition showed that EPS is a heteropolymer, with mannose, xylose, and glucuronic acid identified as the predominant monosaccharides and derivatives. Nuclear magnetic resonance spectroscopy (¹³C and ¹H) confirmed that EPS is a heteropolysaccharide, entirely in α- anomeric configuration, with glucuronic acid as a main monomer that is probably linked to mannose and xylose via α-glycosidic linkages. Bioactivity assessment of EPS revealed that it exhibits antibacterial activity against several strains, notably, Bacillus subtilis (MIC: 0.6 ± 0.05 mg/mL), Bacillus cereus (MIC: 1 ± 0.01 mg/mL), Escherichia coli (MIC: 0.8 ± 0.01 mg/mL) and Klebsiella pneumonia (MIC: 0.8 ± 0.01 mg/mL). Antioxidant activity was measured using the DPPH radical scavenging assay, yielding an IC₅₀ of 6.83 mg/mL. Besides, EPS was also found to exhibit an interesting emulsifying property with several oil types, indicating its potential as a versatile biopolymer for applications in various industrial sectors.

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从盐度胁迫下培养的蓝藻菌株Arthrospira maxima中获得一种新的生物活性和功能性外多糖。

蓝藻胞外多糖（EPS）由蓝藻释放在周围环境中，其主要目的是防止有害的环境条件。近年来，由于其独特的结构特征、功能特性和在各个领域的潜在应用，引起了人们的广泛关注。本研究描述了最大节螺旋藻在盐度胁迫下EPS产量的评价。应用高达40 g/L的高盐度提高了EPS的产量，通过酒精沉淀、膜透析和冻干来收集和纯化EPS。产率为60 mg/L。通过粒径隔离层析，纯化的EPS表观分子量为2.1 × 105 Da。单糖组成表明，EPS为杂多聚物，甘露糖、木糖和葡萄糖醛酸是主要的单糖及其衍生物。核磁共振波谱（13C和1H）证实了EPS是一种杂多糖，完全为α-异头异构体，葡萄糖醛酸为主要单体，可能通过α-糖苷键与甘露糖和木糖相连。生物活性评价表明，EPS对枯草芽孢杆菌（MIC: 0.6±0.05 mg/mL）、蜡样芽孢杆菌（MIC: 1±0.01 mg/mL）、大肠杆菌（MIC: 0.8±0.01 mg/mL）和肺炎克雷伯菌（MIC: 0.8±0.01 mg/mL）等多种菌均有抑菌活性。使用DPPH自由基清除法测量抗氧化活性，得到IC₅0为6.83 mg/mL。此外，研究还发现EPS对几种油具有有趣的乳化特性，这表明它作为一种多功能生物聚合物在各种工业领域的应用潜力。

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来源期刊

Bioprocess and Biosystems Engineering 工程技术-工程：化工

CiteScore

7.90

自引率

2.60%

发文量

147

审稿时长

2.6 months

期刊介绍： Bioprocess and Biosystems Engineering provides an international peer-reviewed forum to facilitate the discussion between engineering and biological science to find efficient solutions in the development and improvement of bioprocesses. The aim of the journal is to focus more attention on the multidisciplinary approaches for integrative bioprocess design. Of special interest are the rational manipulation of biosystems through metabolic engineering techniques to provide new biocatalysts as well as the model based design of bioprocesses (up-stream processing, bioreactor operation and downstream processing) that will lead to new and sustainable production processes. Contributions are targeted at new approaches for rational and evolutive design of cellular systems by taking into account the environment and constraints of technical production processes, integration of recombinant technology and process design, as well as new hybrid intersections such as bioinformatics and process systems engineering. Manuscripts concerning the design, simulation, experimental validation, control, and economic as well as ecological evaluation of novel processes using biosystems or parts thereof (e.g., enzymes, microorganisms, mammalian cells, plant cells, or tissue), their related products, or technical devices are also encouraged. The Editors will consider papers for publication based on novelty, their impact on biotechnological production and their contribution to the advancement of bioprocess and biosystems engineering science. Submission of papers dealing with routine aspects of bioprocess engineering (e.g., routine application of established methodologies, and description of established equipment) are discouraged.