The Photomodification Method Allows for Determining the Composition of the Full and Soft Protein Corona on the Lipid Surface of Composite Nanoparticles.

IF 4.4 3区材料科学 Q2 CHEMISTRY, MULTIDISCIPLINARY Nanomaterials Pub Date : 2024-12-09 DOI:10.3390/nano14231976

Anna V Epanchintseva, Svetlana V Baranova, Julia E Poletaeva, Anastasiya V Tupitsyna, Elena I Ryabchikova, Ilya S Dovydenko

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Abstract

A protein corona (PC) is formed and maintained on the surface of any nanoparticle (NP) introduced into biological media. The full PC is formed by a hard and soft corona, and the latter determines the nature of the interaction of NPs with cells and the body's liquids. Nanomedicines are becoming increasingly important in modern health services, making information about the composition of PCs on the surface of NPs critically important for "managing" the behavior of nano-objects in the body. Currently, only a few studies report on the composition of the complete PC, since the isolation and preservation of the soft corona on the surface of the NP is extremely difficult. Recently, we proposed for the first time a photomodification method to fix PCs on the lipid surface of composite NPs, along with their isolation and purification. In this work, using tandem mass spectrometry, we successively determined the composition of the hard and full corona on the lipid surface of composite NPs, and we also identified the composition of the soft corona. To test the method, we changed the composition of the medium whose proteins formed the soft corona, and we found changes in its composition.

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任何引入生物介质的纳米粒子（NP）表面都会形成并保持一个蛋白质电晕（PC）。完整的 PC 由硬电晕和软电晕组成，后者决定了 NP 与细胞和体内液体相互作用的性质。纳米药物在现代医疗服务中的地位日益重要，因此，有关纳米粒子表面 PC 组成的信息对于 "管理 "纳米物体在体内的行为至关重要。由于分离和保存 NP 表面的软电晕极为困难，目前只有少数研究报告了完整 PC 的组成。最近，我们首次提出了一种将 PC 固定在复合 NP 的脂质表面并进行分离和纯化的光修饰方法。在这项工作中，我们利用串联质谱法先后测定了复合 NPs 脂质表面硬冠和全冠的成分，并确定了软冠的成分。为了测试该方法，我们改变了蛋白质形成软电晕的介质的成分，结果发现其成分发生了变化。

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来源期刊

Nanomaterials NANOSCIENCE & NANOTECHNOLOGY-MATERIALS SCIENCE, MULTIDISCIPLINARY

CiteScore

8.50

自引率

9.40%

发文量

3841

审稿时长

14.22 days

期刊介绍： Nanomaterials (ISSN 2076-4991) is an international and interdisciplinary scholarly open access journal. It publishes reviews, regular research papers, communications, and short notes that are relevant to any field of study that involves nanomaterials, with respect to their science and application. Thus, theoretical and experimental articles will be accepted, along with articles that deal with the synthesis and use of nanomaterials. Articles that synthesize information from multiple fields, and which place discoveries within a broader context, will be preferred. There is no restriction on the length of the papers. Our aim is to encourage scientists to publish their experimental and theoretical research in as much detail as possible. Full experimental or methodical details, or both, must be provided for research articles. Computed data or files regarding the full details of the experimental procedure, if unable to be published in a normal way, can be deposited as supplementary material. Nanomaterials is dedicated to a high scientific standard. All manuscripts undergo a rigorous reviewing process and decisions are based on the recommendations of independent reviewers.