PsHB7/12 gene participated in the overwintering process of Pyrus sinkiangensis through negative feedback regulation of ABA.

IF 6.1 2区生物学 Q1 PLANT SCIENCES Plant Physiology and Biochemistry Pub Date : 2024-12-26 DOI:10.1016/j.plaphy.2024.109440

Xiaoyan Liu, Aowei Li, Xueying Yang, Guanghong Luo, Jianbo Zhu

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Abstract

Pyrus sinkiangensis, a crucial economic fruit tree in Xinjiang, China, experiences winter hardiness that significantly influences its yield and fruit quality. This study aimed to investigate the role of PsHB7/12 in cold resistance of Pyrus sinkiangensis and its regulation of abscisic acid (ABA) signaling. Through physiological assessments and transcriptome analysis, we identified a peak expression of PsHB7/12 in January, which was strongly induced by ABA. We found a correlation between ABA concentrations and changes in water content and soluble protein levels during overwintering process. Further analysis of yeast one-hybrid and the luciferase assay revealed that PsHB7/12 was involved in the negative regulation of ABA signal by inhibiting the expression of PsPYL4. Additionally, the overexpression of the PsHB7/12 may have complex effects on ABA signaling through the modulation of expression of members of the PsPP2Cs family. In summary, PsHB7/12 regulates the ABA signaling pathway through a negative feedback mechanism. These findings reveal the critical role of PsHB7/12 in cold stress adaptation in Pyrus sinkiangensis and provide new molecular markers for breeding stress-resistant fruit trees.

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PsHB7/12基因通过ABA的负反馈调控参与了新疆梨的越冬过程。

新疆梨（Pyrus sinkiangensis）是新疆重要的经济果树，其抗寒性显著影响其产量和果实品质。本研究旨在探讨PsHB7/12基因在新疆梨（Pyrus sinkiangensis）抗寒中的作用及其对ABA信号的调控作用。通过生理评估和转录组分析，我们发现PsHB7/12在1月份达到表达高峰，受到ABA的强烈诱导。我们发现，在越冬过程中，ABA浓度与水分含量和可溶性蛋白水平的变化存在相关性。进一步的酵母单杂交分析和荧光素酶分析表明，PsHB7/12通过抑制PsPYL4的表达参与ABA信号的负调控。此外，PsHB7/12的过表达可能通过调节pspp2c家族成员的表达而对ABA信号传导产生复杂的影响。综上所述，PsHB7/12通过负反馈机制调控ABA信号通路。这些发现揭示了PsHB7/12基因在新疆梨冷胁迫适应中的重要作用，为培育抗逆性果树提供了新的分子标记。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Plant Physiology and Biochemistry 生物-植物科学

CiteScore

11.10

自引率

3.10%

发文量

410

审稿时长

33 days

期刊介绍： Plant Physiology and Biochemistry publishes original theoretical, experimental and technical contributions in the various fields of plant physiology (biochemistry, physiology, structure, genetics, plant-microbe interactions, etc.) at diverse levels of integration (molecular, subcellular, cellular, organ, whole plant, environmental). Opinions expressed in the journal are the sole responsibility of the authors and publication does not imply the editors'' agreement. Manuscripts describing molecular-genetic and/or gene expression data that are not integrated with biochemical analysis and/or actual measurements of plant physiological processes are not suitable for PPB. Also "Omics" studies (transcriptomics, proteomics, metabolomics, etc.) reporting descriptive analysis without an element of functional validation assays, will not be considered. Similarly, applied agronomic or phytochemical studies that generate no new, fundamental insights in plant physiological and/or biochemical processes are not suitable for publication in PPB. Plant Physiology and Biochemistry publishes several types of articles: Reviews, Papers and Short Papers. Articles for Reviews are either invited by the editor or proposed by the authors for the editor''s prior agreement. Reviews should not exceed 40 typewritten pages and Short Papers no more than approximately 8 typewritten pages. The fundamental character of Plant Physiology and Biochemistry remains that of a journal for original results.