Megan L Schaller, Madeline M Sykes, Sarah A Easow, Faith R Carranza, Angela M Tuckowski, Yatrik M Shah, Scott F Leiser
{"title":"Perception of <i>Enterococcus faecalis</i> without infection induces <i>fmo-2</i> in <i>C. elegans</i>.","authors":"Megan L Schaller, Madeline M Sykes, Sarah A Easow, Faith R Carranza, Angela M Tuckowski, Yatrik M Shah, Scott F Leiser","doi":"10.17912/micropub.biology.001422","DOIUrl":null,"url":null,"abstract":"<p><p><i>C. elegans</i> pathogenic susceptibility is influenced by the worm's detection of its environment and its capacity to resist and resolve damage following infection. Here, we use a model where worms can sense, but not ingest, the pathogen <i>Enterococcus faecalis</i> (EF) <i>.</i> We identify that perception of EF without infection induces the stress-response gene <i>fmo-2. We</i> further identify that neural and intestinal signaling genes are necessary for <i>fmo-2</i> induction without active infection. Finally, we show that <i>fmo-2</i> overexpression is sufficient to extend lifespan with EF exposure, while <i>fmo-2</i> KO is not detrimental, suggesting that additional <i>fmo-2</i> expression benefits worms in this condition.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11759934/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"microPublication biology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.17912/micropub.biology.001422","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/1/1 0:00:00","PubModel":"eCollection","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
C. elegans pathogenic susceptibility is influenced by the worm's detection of its environment and its capacity to resist and resolve damage following infection. Here, we use a model where worms can sense, but not ingest, the pathogen Enterococcus faecalis (EF) . We identify that perception of EF without infection induces the stress-response gene fmo-2. We further identify that neural and intestinal signaling genes are necessary for fmo-2 induction without active infection. Finally, we show that fmo-2 overexpression is sufficient to extend lifespan with EF exposure, while fmo-2 KO is not detrimental, suggesting that additional fmo-2 expression benefits worms in this condition.