Eu-Doped TiO2 Coatings via One-Step In Situ Preparation Enhance Macrophage Polarization and Osseointegration of Implants.

Abstract

The controllable regulation of immune and osteogenic processes plays a critical role in the modification of biocompatible materials for tissue regeneration. In this study, titanium dioxide-europium coatings (MAO/Eu) were prepared on the surface of a titanium alloy (Ti-6Al-4V) via a one-step process combining microarc oxidation (MAO) and in situ doping. The incorporation of Eu significantly improved the hydrophilic and mechanical properties of the TiO₂ coatings without altering their morphology. The presence of Eu effectively stimulated calcium influx in macrophages and activated β-catenin through the wnt/β-catenin signaling pathway. Consequently, macrophage M2 polarization was accelerated through the overexpression of prostaglandin E2 (PGE2). Additionally, Ca²⁺ promoted the osteogenic differentiation of MC3T3-E1 cells through the synergistic upregulation of transcription factors (e.g., AP-1, BMP-2). In vivo studies demonstrated that MAO/Eu coatings significantly enhanced osseointegration compared with the titanium alloy group. Therefore, MAO/Eu shows promising potential as an ideal coating for implants that offers effective immunomodulatory strategies and improves bone integration.