Rapid bacterial detection through microfluidic integration with a glucometer

IF 4.8 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Bioelectrochemistry Pub Date : 2025-02-06 DOI:10.1016/j.bioelechem.2025.108936

Merve Eryilmaz , Sibel Ilbasmis-Tamer , Sallahuddin Panhwar , Emine Kübra Tayyarcan , İsmail Hakkı Boyaci , Zekiye Suludere , Demet Çetin , Adem Zengin , Ender Yıldırım , Uğur Tamer

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Abstract

We present a novel approach for sensitive and portable detection of pathogenic bacteria, which is crucial for household and clinical practice. Our method employs immunoliposomes, antibodies, and microchip to detect specific pathogens quantitatively. Gold and metal metal–organic nanoparticles and liposomes were characterized using high-resolution techniques like TEM and SEM. Utilizing a commercial, personal glucose meter (PGM), we initially detected released glucose from antibody-modified liposomes and microchips with MOF-NPs. Detection on the microchip was achieved within 30 min, while the PGM analysis took only one minute for targeted bacteria, yielding glucose signals of 66 mg/dL and 69 mg/dL, respectively. Serial dilutions with group A-Streptococcus pyogenes (GAS) (1.4 × 10^{^4}–1.4 × 10^{^8} CFU/mL) demonstrated quantitative measurement applicability. This innovative approach and a portable PGM hold promise for various industries, including physician labs, hospitals, and households.

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来源期刊

Bioelectrochemistry 生物-电化学

CiteScore

9.10

自引率

6.00%

发文量

238

审稿时长

38 days

期刊介绍： An International Journal Devoted to Electrochemical Aspects of Biology and Biological Aspects of Electrochemistry Bioelectrochemistry is an international journal devoted to electrochemical principles in biology and biological aspects of electrochemistry. It publishes experimental and theoretical papers dealing with the electrochemical aspects of: • Electrified interfaces (electric double layers, adsorption, electron transfer, protein electrochemistry, basic principles of biosensors, biosensor interfaces and bio-nanosensor design and construction. • Electric and magnetic field effects (field-dependent processes, field interactions with molecules, intramolecular field effects, sensory systems for electric and magnetic fields, molecular and cellular mechanisms) • Bioenergetics and signal transduction (energy conversion, photosynthetic and visual membranes) • Biomembranes and model membranes (thermodynamics and mechanics, membrane transport, electroporation, fusion and insertion) • Electrochemical applications in medicine and biotechnology (drug delivery and gene transfer to cells and tissues, iontophoresis, skin electroporation, injury and repair). • Organization and use of arrays in-vitro and in-vivo, including as part of feedback control. • Electrochemical interrogation of biofilms as generated by microorganisms and tissue reaction associated with medical implants.