{"title":"Improvement of gain and spatial resolution for impulsive stimulated Brillouin scattering microscopy","authors":"Taoran Le, Jiarui Li, Haoyun Wei, Yan Li","doi":"10.1016/j.pacs.2025.100696","DOIUrl":null,"url":null,"abstract":"<div><div>Brillouin microscopy has been widely used in the mechanical imaging of cells and tissues, and the signal-to-noise (SNR) ratio limits the spectral integration time. Impulsive stimulated Brillouin scattering (ISBS) microscopy is a new elastic imaging technique. As a variant of stimulated Brillouin scattering (SBS), ISBS can overcome the weak signal of spontaneous Brillouin scattering. A simple model can estimate SBS gain. However, the theoretical ISBS gain has not been compared with SBS gain. This paper gives the theoretical ISBS gain estimation, and experiments are designed to verify estimation reliability. The heterodyne ISBS gain coefficient can be much higher than SBS gain coefficient. The relationship between ISBS gain coefficient and spatial resolution is then discussed. We anticipate that the ISBS setup optimization can improve spatial resolution and gain, potentially enabling fast and high spatial resolution imaging of biological cells.</div></div>","PeriodicalId":56025,"journal":{"name":"Photoacoustics","volume":"42 ","pages":"Article 100696"},"PeriodicalIF":7.1000,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Photoacoustics","FirstCategoryId":"5","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2213597925000151","RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENGINEERING, BIOMEDICAL","Score":null,"Total":0}
引用次数: 0
Abstract
Brillouin microscopy has been widely used in the mechanical imaging of cells and tissues, and the signal-to-noise (SNR) ratio limits the spectral integration time. Impulsive stimulated Brillouin scattering (ISBS) microscopy is a new elastic imaging technique. As a variant of stimulated Brillouin scattering (SBS), ISBS can overcome the weak signal of spontaneous Brillouin scattering. A simple model can estimate SBS gain. However, the theoretical ISBS gain has not been compared with SBS gain. This paper gives the theoretical ISBS gain estimation, and experiments are designed to verify estimation reliability. The heterodyne ISBS gain coefficient can be much higher than SBS gain coefficient. The relationship between ISBS gain coefficient and spatial resolution is then discussed. We anticipate that the ISBS setup optimization can improve spatial resolution and gain, potentially enabling fast and high spatial resolution imaging of biological cells.
PhotoacousticsPhysics and Astronomy-Atomic and Molecular Physics, and Optics
CiteScore
11.40
自引率
16.50%
发文量
96
审稿时长
53 days
期刊介绍：
The open access Photoacoustics journal (PACS) aims to publish original research and review contributions in the field of photoacoustics-optoacoustics-thermoacoustics. This field utilizes acoustical and ultrasonic phenomena excited by electromagnetic radiation for the detection, visualization, and characterization of various materials and biological tissues, including living organisms.
Recent advancements in laser technologies, ultrasound detection approaches, inverse theory, and fast reconstruction algorithms have greatly supported the rapid progress in this field. The unique contrast provided by molecular absorption in photoacoustic-optoacoustic-thermoacoustic methods has allowed for addressing unmet biological and medical needs such as pre-clinical research, clinical imaging of vasculature, tissue and disease physiology, drug efficacy, surgery guidance, and therapy monitoring.
Applications of this field encompass a wide range of medical imaging and sensing applications, including cancer, vascular diseases, brain neurophysiology, ophthalmology, and diabetes. Moreover, photoacoustics-optoacoustics-thermoacoustics is a multidisciplinary field, with contributions from chemistry and nanotechnology, where novel materials such as biodegradable nanoparticles, organic dyes, targeted agents, theranostic probes, and genetically expressed markers are being actively developed.
These advanced materials have significantly improved the signal-to-noise ratio and tissue contrast in photoacoustic methods.
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