Mammaglobin-A as a Diagnostic Marker of Canine Mammary Tumors: Insights from Immunohistochemical Analysis

IF 1.3 4区综合性期刊 Q3 MULTIDISCIPLINARY SCIENCES National Academy Science Letters Pub Date : 2024-09-28 DOI:10.1007/s40009-024-01444-x

Shikha Sharma, B. V. Sunil Kumar, Kuldip Gupta, Ashwani Kumar

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Abstract

Mammaglobin-A (MAM-A) is a glycoprotein predominantly linked to mammary tissue. It is a secretory protein, characterized by its composition of 93 amino acids and a molecular weight of around 10 kDa. The primary objective of this study is to evaluate the tissue expression of MAM-A in different histotypes of canine mammary tumors (CMT). The present study includes a total of 40 samples of CMT and 5 samples of apparently healthy canine mammary tissues, which were subjected to histological and immunohistochemical examinations. Hyperimmune serum was generated against recombinant MAM-A (rMAM-A) in rabbits to establish a standardized immunohistochemical protocol for evaluating the expression of MAM-A protein in different histotypes of CMT. The histological study demonstrated a wide array of tumor classifications, predominantly consisting of carcinoma, followed by carcinosarcoma and sarcoma. The immunohistochemical examination demonstrated a pronounced expression of MAM-A in the majority of CMT histotypes. The immunohistochemical expression of MAM-A in CMT indicates its potential as a valuable diagnostic biomarker.

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作为犬乳腺肿瘤诊断标记物的 Mammaglobin-A：免疫组化分析的启示

mammagloin - a （MAM-A）是一种主要与乳腺组织相关的糖蛋白。它是一种分泌蛋白，其特点是由93个氨基酸组成，分子量约为10 kDa。本研究的主要目的是评估MAM-A在犬乳腺肿瘤（CMT）不同组织类型中的组织表达。本研究共选取40例CMT标本和5例表面健康的犬乳腺组织标本，进行组织学和免疫组化检查。制备兔重组MAM-A （rmm - a）超免疫血清，建立标准化的免疫组化方案，评估不同组织型CMT中MAM-A蛋白的表达。组织学研究显示多种肿瘤分类，主要是癌，其次是癌肉瘤和肉瘤。免疫组化检查显示在大多数CMT组织型中有明显的MAM-A表达。MAM-A在CMT中的免疫组织化学表达表明其作为一种有价值的诊断生物标志物的潜力。

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来源期刊

National Academy Science Letters 综合性期刊-综合性期刊

CiteScore

2.20

自引率

0.00%

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审稿时长

12 months

期刊介绍： The National Academy Science Letters is published by the National Academy of Sciences, India, since 1978. The publication of this unique journal was started with a view to give quick and wide publicity to the innovations in all fields of science